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MetabolomicsThe Manchurian Walnut Genome: Insights into Juglone and Lipid Biosynthesis

BACKGROUND: Manchurian walnut (Juglans mandshurica Maxim.) is a tree with multiple industrial uses and medicinal properties in the Juglandaceae family (walnuts and hickories). J. mandshurica produces juglone, which is a toxic allelopathic agent and has potential utilization value...

2022-05-18 | MTBLS3657 | MetaboLights
MetabolomicsIntegrated transcriptomic and metabolic profiling reveals the molecular mechanism of improved nitrogen metabolism in walnut (Juglans regia L.) roots mediated by soybean intercropping

The practice of intercropping young walnut orchards with soybeans has emerged as a promising approach within the framework of sustainable agricultural management. However, the precise impacts of soybean intercropping on nitrogen metabolism in walnut roots remain insufficiently elucidated. This st...

2026-01-19 | MTBLS13719 | MetaboLights
MetabolomicsTwo UGT84A Family Glycosyltransferases Regulate Phenol, Flavonoid, and Tannin Metabolism in Juglans regia (English Walnut)
We showed previously that gallic acid is produced in walnut from 3-dehydroshikimate by a shikimate dehydrogenase (JrSkDH). This study focuses on the next step in the hydrolysable tannin pathway, the formation of 1-O-galloyl-β-D-glucose from the phenolic gallic acid and UDP glucose by a glycos...
2022-10-11 | MTBLS2042 | MetaboLights
MetabolomicsWalnut protein hydrolysate alleviates neuroinflammation and memory impairment in naturally aged mice via a gut microbiota-metabolite-neuroinflammation axis
This study investigated age-associated alterations in the serum metabolome and the metabolic effects of walnut protein hydrolysate (WPH) supplementation in naturally aged mice. Male C57BL/6J mice at different ages (2-month, 10-month and 18-month) were used to characterize metabolic changes associate...
2026-09-04 | MTBLS15363 | MetaboLights
MetabolomicsExtracellular Vesicles from probiotics mediated Walnut green husk polysaccharide alleviates ochratoxin-A-induced cognitive impairment via SCFA and 5-HT mediated microbiota-gut-brain axis

BACKGROUND: Ochratoxin A (OTA) have made it a serious hazard to food safety worldwide that poses a serious health risk to humans. Our previous research showed that Walnut green husk polysaccharides (WGP) can effectively alleviate intestinal inflammation and intestinal microbiota ...

2024-10-10 | MTBLS10673 | MetaboLights
ProteomicsDiscovery, Verification, and Validation of Walnut Protein Marker Peptides Using LC-MS/MS Approaches
A key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic food protein analysis methods is to use protein marker peptides with good analytical performances in LC-MS analysis of commercial processed foods. In this study, we developed a multistage walnut protein marker pept...
ORGANISM(S): Juglans regia (English walnut) 
2023-07-31 | PXD043010 | Pride
Food allergy Lc-ms Validation Walnut marker peptide 
ProteomicsProteomic study of walnut oleosome and first evidence on oleosinsensitization in allergic patients
The research project aims to investigate the walnut oleosome sub-proteome of raw, boiled, and roasted kernels. To achieve this goal both off-gel and in-gel approaches were used. Moreover, the immunoblotting experiments were performed to characterize the walnut OB proteins as potential allergens by d...
ORGANISM(S): Juglans regia (English walnut) 
2023-05-18 | PXD038199 | Pride
Processing Walnut Oleosome Sub-proteome Oleosin Food allergy Oil body 
ProteomicsPurification and characterization of a novel allergenic walnut vicilin-like protein
Walnuts are potent inducers of food allergic symptoms. So far, five allergens were identified, among those, Jug r 2 belonging to the vicilin family. Based on the identification of an IgE-reactive clone from a cDNA library in 1999 (Teuber et al.), the sequence fragment of Jug r 2 was determined. Howe...
ORGANISM(S): Juglans regia (English walnut) 
2018-10-17 | PXD005744 | Pride
Vicilin Walnut Food allergy 
ProteomicsRoasted English Walnut Label-Free LC-MS/MS Quantification
Thermal processing of allergenic foods has the potential to induce a number of changes in the constituent allergens, but many of the effects of heat treatment are poorly defined. Like numerous other commonly allergenic tree nuts, walnuts often undergo heat treatment, such as roasting, prior to consu...
ORGANISM(S): Juglans regia (English walnut) 
2016-05-18 | PXD000907 | Pride
Food allergens Walnut Thermal processing 
ProteomicsLow Molecular Weight Allergens from English Walnut (Juglans Regia)
Low molecular weight (LMW) proteins were purified from English walnuts (J. regia cv. Chandler). The LMW proteins were separated by anion exchange chromatography. The anion exchange fractions were analyzed by SDS-PAGE, and protein bands were excised. In gel trypsin digestion was conducted (following ...
ORGANISM(S): Juglans regia (English walnut) 
2019-11-28 | PXD000634 | Pride
Food allergen Tree nut Vicilin Walnut 
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