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Hepatocellular carcinoma (HCC) is susceptible to early recurrence and lacks effective predictive markers. Mass Spectrometry (MS)-based proteomics has emerged as a potent tool for identifying cancer biomarker profiles in various bodily fluids. In this study, we established a MS-based biomarker discov...
ORGANISM(S): Homo Sapiens 
2023-12-15 | PXD047852 |
Hepatocellular Carcinoma(HCC)is one of the most common malignat tumor,which is causing the second leading cancer-related death worldwide.
ORGANISM(S): Homo Sapiens 
Cell samples were lysed in lysis buffer (8 M urea, 1% protease inhibitor cocktail). The supernatant was collected and the protein concentration was determined with BCA kit according to the manufacturer’s instructions. The peptides were obtained by digesting the protein with trypsin. Then, peptides w...
ORGANISM(S): Rattus Norvegicus 
To find out the potential interacting proteins of Asb10 in cardiomyocytes, we conducted the IP-MS in NRVMs overexpressing Asb10.
ORGANISM(S): Rattus Norvegicus 
2025-03-07 | PXD061568 |
As for gastrocnemius (CKD vs. NC =4 vs. 4), the protein was extracted by homogenate and SDT lysis. Next, we did the following steps: SDS-PAGE separation, Filter-aided sample preparation (FASP Digestion), TMT labeling, peptide fractionation with reversed-phase (RP) chromatography and mass spectrometr...
ORGANISM(S): Mus Musculus 
Protein mass spectrum analysis of kidney tissue from CKD and NC mice
ORGANISM(S): Mus Musculus 
As for serum (CKD vs. NC =4 vs. 4), the protein was extracted by homogenate and SDT lysis. We utilized immunoaffinity depletion of serum high-abundance proteins. Next, we did the following steps: SDS-PAGE separation, Filter-aided sample preparation (FASP Digestion), TMT labeling, peptide fractionati...
ORGANISM(S): Mus Musculus 
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