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Three apoptosis inducing proteins, ACO1, STK3 and XBP1 were discovered in a previous reverse transfection screen. HEK293T cells were harvested and RNA extracted from the cells after over-expression of these 3 proteins at 12, 24 and 48 hours. A positive apoptosis inducer staurosporine and a negative ...
ORGANISM(S): Homo sapiens 
Untargeted metabolomics is an established approach in toxicology for characterising endogenous metabolic responses to xenobiotic exposure. Detecting the xenobiotic and its biotransformation products as part of the metabolomics analysis provides an opportunity to simultaneously gain deep insights int...
2023-07-03 | MTBLS2746 | MetaboLights
We employed a massively parallel, high resolution Capture-C based method to simultaneously characterize the genome-wide interactions of all human promoters in any cell type. We applied this approach to study the promoter interactome of HepG2 cells (3 biological replicates). We designed a custom Agil...
ORGANISM(S): Homo sapiens 
The forkhead DNA-binding protein FOXP3 is critical for the development and suppressive function of CD4+CD25+ regulatory T cells (TREG), which play a key role in maintaining self tolerance. Functionally, FOXP3 is capable of repressing transcription of cytokine genes regulated by the Nuclear Factor of...
ORGANISM(S): Homo sapiens 
The use of custom-made focused microarrays has become a popular alternative, allowing the study of genes relevant to a specific hypothesis. To address the limitations of commercial arrays in toxicogenomics investigations, we have developed a custom mouse oligonucleotide microarray, the HC ToxArrayTM...
ORGANISM(S): Mus musculus 
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