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TranscriptomicsMethylation profiling of normal and cancerous human breast samples
To assess changes in DNA methylation state associated with breast cancer we analyzed DNA from normal and cancerous breast specimens on CpG islands microarray using methylation-sensitive enzyme McrBC for target preparation. Cy3 was used for McrBC cut sample, Cy5 was used for reference Mock sample.
ORGANISM(S): Homo sapiens 
2006-11-03 | E-MEXP-881 | biostudies-arrayexpress
TranscriptomicsMethylation profiling of normal and cancerous breast cells from human patients and cell lines in a 125 kB region of the HOXA cluster
To evaluate DNA methylation profile associated with breast cancer in 125 kB region of HOXA cluster we analyzed DNA from normal cancerous breast specimens and cell lines. Analysis was performed on the tiling array covering entire HOXA region with 500bp resolution. Immunoprecipitation with anti-methyl...
ORGANISM(S): Homo sapiens 
2006-11-03 | E-MEXP-880 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of honey bee brain and ovary reveals pheromone response is correlated with reproductive traits and brain gene expression in individual honey bees
In honey bees (Apis mellifera), the reproductive queen produces a pheromonal signal that regulates many aspects of worker behavior and physiology and is critical for maintaining colony organization. Queen mandibular pheromone (QMP) inhibits worker reproduction, attracts workers from a short distanc...
ORGANISM(S): Apis mellifera 
2008-12-31 | E-MEXP-1724 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of honey bee queens Apis mellifera L. to investigate the differential effects of insemination volume and substance on reproductive changes
Mating causes dramatic changes in female insects at the behavioral, physiological, and molecular levels. The factors driving these changes (e.g., seminal proteins, seminal volume) and the molecular pathways by which these factors are operating have been characterized only in a handful of insect spe...
ORGANISM(S): Apis mellifera 
2013-05-21 | E-MEXP-3681 | biostudies-arrayexpress
TranscriptomicsThe Epstein-Barr virus induced tumor suppressor miR-34a is growth promoting in EBV-infected B cells
Epstein-Barr virus (EBV) infection of primary human B cells drives their indefinite proliferation into lymphoblastoid cell lines (LCLs). B cell immortalization depends on expression of viral latency genes as well as the regulation of host genes. Given the important role of miRNAs in regulating fund...
ORGANISM(S): Homo sapiens 
2012-04-19 | E-GEOD-36926 | biostudies-arrayexpress
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