Sort   by:  
 Page size 
TranscriptomicsThe Exoribonuclease Nibbler Controls 3' End Processing of MicroRNAs in Drosophila
MicroRNAs (miRNAs) are endogenous noncoding small RNAs with important roles in many biological pathways; their generation and activity are under precise regulation. Emerging evidence suggests that miRNA pathways are precisely modulated with controls at the level of transcription, processing, and sta...
ORGANISM(S): Drosophila melanogaster 
2011-11-17 | E-GEOD-31689 | biostudies-arrayexpress
GenomicsDevelopmental Regulation and Individual Differences of Neuronal Epigenomes in the Prefrontal Cortex
We report global distribution of trimethylated Histone H3 Lysine 4 (H3K4me3) in human prefrontal cortex neurons at different ages Neuronal nuclei from human prefrontal cortex were isolated by FACS. Regions marked by H3K4me3 were identified by chromatin immunoprecipiation followed by deep sequencing.
ORGANISM(S): Homo sapiens 
2010-04-20 | E-GEOD-21172 | biostudies-arrayexpress
TranscriptomicsSorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway
In flies, small silencing RNAs are sorted between Argonaute1 (Ago1), the central protein component of the microRNA (miRNA) pathway, and Argonaute2 (Ago2), which mediates RNA interference. Extensive double-stranded characterM-bM-^@M-^Tas is found in small interfering RNAs (siRNAs)M-bM-^@M-^Tdirects d...
ORGANISM(S): Drosophila melanogaster 
2009-10-31 | E-GEOD-18806 | biostudies-arrayexpress
TranscriptomicsDicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals
Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. loquacious (loqs) encodes three Dicer partner proteins, Loqs-PA, Loqs-PB, and, Loqs-PD, generated by alternative splicing. To understand the function of each Loqs...
ORGANISM(S): Mus musculus 
2012-12-07 | E-GEOD-37443 | biostudies-arrayexpress
TranscriptomicsExploring the DNA-Recognition Potential of Homeodomains
We utilized a bacterial selection system to isolate Engrailed variants from a randomized library that are compatible with each of the 64 possible 3’ triplet sites (i.e. TAANNN). The DNA-binding specificity of 151 representative HD variants from these populations was subsequently characterized. Ma...
ORGANISM(S): synthetic construct 
2012-02-15 | E-GEOD-35806 | biostudies-arrayexpress
GenomicsWhole genome DNAse hypersensitivity in human CD4+ T-cells
Mapping DNaseI hypersensitive (HS) sites is an accurate method of identifying the location of genetic regulatory elements, including promoters, enhancers, silencers, insulators, and locus control regions. We employed whole genome tiled array strategies to identify DNaseI HS sites within human prima...
ORGANISM(S): Homo sapiens 
2007-11-19 | E-GEOD-8486 | biostudies-arrayexpress
GenomicsHdac6 regulates Tip60-p400 function in stem cells (sequencing)
The Tip60 (also known as Kat5) lysine acetyltransferase functions broadly as a transcriptional co-activator that acetylates histones. In contrast, Tip60 functions in embryonic stem cells (ESCs) both to silence genes that promote differentiation and to activate genes required for proliferation. The m...
ORGANISM(S): Mus musculus 
2013-11-20 | E-GEOD-42209 | biostudies-arrayexpress
GenomicsH3K4me3 modification profiling of Huntingtons disease and control human post-mortem prefrontal cortex brain samples
Huntington’s disease (HD) is an autosomal-dominant neurodegenerative disorder resulting from expansion in the number of CAG repeats in the coding region of exon 1 of the Huntingtin (HTT) gene. One of the most widely studied chromatin modifications is trimethylated lysine 4 of histone 3 (H3K4me3). ...
ORGANISM(S): Homo sapiens 
2015-12-14 | E-GEOD-68952 | biostudies-arrayexpress
TranscriptomicsEndogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA as a defense against viral infection. Here, we identify 21-nt long, endogenous siRNAs (endo-siRNAs) corresponding to transposons and heterochromatic...
ORGANISM(S): Drosophila melanogaster 
2008-04-10 | E-GEOD-11019 | biostudies-arrayexpress
TranscriptomicsPaternally-induced transgenerational environmental reprogramming of metabolic gene expression in mammals (Affymetrix)
Epigenetic information can be inherited through the mammalian germline, and represents a plausible transgenerational carrier of environmental information. To test whether transgenerational inheritance of environmental information occurs in mammals, we carried out an expression profiling screen for g...
ORGANISM(S): Mus musculus 
2011-01-14 | E-GEOD-25896 | biostudies-arrayexpress
Sort   by:  
 Page size