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MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
MetabolomicsSingle-cell metabolic fingerprints discover a cluster of circulating tumor cells with distinct metastatic potential

Circulating tumor cells (CTCs) are recognized as direct seeds of metastasis. However, CTC count may not be the 'best' indicator of metastatic risk because their heterogeneity is generally neglected. In this study, we develop a molecular typing system to predict colorectal cancer metastasis potent...

2023-08-21 | MTBLS5028 | MetaboLights
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsMus musculus primary CD4+ T cell di-glycine remnant and whole cell proteomics
It is known that ubiquitination is important for T cell receptor (TCR) signaling during T cell activation but the breadth of ubiquitination events triggered during TCR signaling is not completely understood. This dataset utilizes di-glycine remnant profiling combined with mass spectrometry to identi...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-09 | PXD012831 | Pride
Mus musculus primary cd4+ t cell di-glycine remnant and whole cell proteomics 
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2024-09-10 | PXD027459 | JPOST Repository
ProteomicsKM-12 Whole Cell Lysate Proteomics
The KM-12 cell line, representative of colorectal cancer indications, was used to explore the effect that multiple compounds would have in the profile of transcription factor occupancy. Cells were dosed with 3 concentrations of each of the 5 compounds provided for 3 different timepoints. Both a chro...
ORGANISM(S): Homo sapiens (Human) 
2026-04-06 | PXD065035 | Pride
Lc-msms Colorectal cancer Timstof 
ProteomicsHypoxia-induced changes in the fibroblast secretome, exosome, and whole-cell proteome using cultured, cardiac-derived cells isolated from neonatal mice
Dataset Pertaining to the proteome changes of the cardiac fibroblast in the whole cell, exosome, and soluble secretome.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2016-08-12 | MSV000080038 | MassIVE
ProteomicsAnalysis of isotopically depleted E. coli MG1655 whole cell lysate
Analysis of whole cell lysate from E. coli MG1655 grown in isotopically depleted M9 minimal medium. Medium was isotopically depleted by formulation of the medium with glucose and ammonium sulfate depleted of carbon-13 and nitrogen-15, respectively. Dataset is licensed under the terms of the Creative...
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2022-01-13 | MSV000088679 | MassIVE
no omics definedmRNA expression profiling of human mesenchymal stromal cell samples grown in vitro under different conditions (foetal calf serum (FCS) based medium versus human platelet lysate (PL) based medium)
Genetic profiling after stem cell culturing. Culturing cells from normal donors, grown in 2 culture media
ORGANISM(S): Homo sapiens 
2023-03-01 | E-MTAB-12272 | biostudies-arrayexpress
ProteomicsProbing the Sensitivity of the Lumos Mass Spectrometer by analyzing varying amounts of whole cell extracts
PROJECT DESCRIPTION The Orbitrap Fusion Lumos mass spectrometer has recently been shown to have better fragmentation and detection than its predecessors. Previous studies using a HeLa digest analyzed on the Lumos mass spectrometer have reported the number of peptides and proteins identified by rever...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2018-04-19 | MSV000082296 | MassIVE
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