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MetabolomicsThermal Profiling Maps Multilayered Organization of the Mitochondrial Proteome

Mitochondria execute essential metabolic, biosynthetic, and signaling functions, yet the regulatory principles governing their proteome remain incompletely understood. Here, we develop a thermal proteome profiling (TPP) workflow applied directly to purified mitochondria, enabling systematic analy...

2025-12-08 | MTBLS7386 | MetaboLights
MetabolomicsProteome allocations change linearly with the specific growth rate of Saccharomyces cerevisiae under glucose limitation

Saccharomyces cerevisiae is a widely used cell factory; therefore, it is important to understand how it organizes key functional parts when cultured under different conditions. Here, we perform a multiomics analysis of S. cerevisiae by culturing the strain with a wide range of s...

2022-08-31 | MTBLS697 | MetaboLights
MetabolomicsIntegrated proteome and lipidome analyses place OCIAD1 at mitochondria-peroxisome intersection balancing lipid metabolism

OCIAD1 (Ovarian Cancer Immunoreactive Antigen Domain Containing 1) is a membrane protein largely localized to mitochondria, however, its function in health or disease is not well understood. To comprehensively characterize the molecular changes upon lack of OCIAD1, we used mass spectrometry to st...

2025-04-25 | MTBLS12329 | MetaboLights
MetabolomicsNon-targeted in vitro metabolomics for proteome-scale identification of novel enzymes in Escherichia coli
Many yet undiscovered enzymes still limit our understanding of metabolism. Here, we develop a high-throughput mass spectrometry approach to comprehensively profile proteome-scale protein collections for in vitro enzymatic activity. Overexpressed or purified proteins are incubated in a supplemented m...
2016-01-11 | MTBLS373 | MetaboLights
MetabolomicsMetabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets

In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves...

2020-02-20 | MTBLS1045 | MetaboLights
ProteomicsUnderstanding early AutoAb signatures in Pituitary Adenomas using CDI HuProt™ (Human Proteome Microarray)
CDI HuProt™ (Human Proteome Microarray), was used to study Pituitary Adenomas PAs (Acromegaly, Cushing's and NFPA) using serum samples, from around 14 individuals (4 Healthy control, 4 Acromegaly, 3 Cushing’s and 3 NFPAs patient samples) were used study their autoantibody profiles. Patient serum sam...
ORGANISM(S): Homo sapiens 
2022-09-30 | E-MTAB-11947 | biostudies-arrayexpress
ProteomicsReanalysis of Pandey lab's draft of the human proteome
The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides was lacking. To this end, Akhilesh Pandey's lab reported a draft map of the human proteome based on...
ORGANISM(S): Homo sapiens 
2015-03-10 | E-PROT-1 | biostudies-arrayexpress
TranscriptomicsA proteomic view on the cell biology of Staphylococcus aureus proteome
The genome sequence is the “blue-print of life”, but proteomics is required to relate this blue-print of life to the actual physiology of living cells. Because of their low complexity – only about 2.000 proteins make a Staphylococcus aureus cell viable – bacteria are excellent model systems to ident...
ORGANISM(S): Staphylococcus aureus 
2009-11-11 | E-GEOD-15060 | biostudies-arrayexpress
ProteomicsProfiling antibody responses to M. tuberculosis proteins in TB suspects’ sera using full-proteome microarrays.
Understanding the immune response to tuberculosis requires greater knowledge of humoral responses. To characterize antibody targets and the effect of disease parameters on target recognition, we developed a systems immunology approach that integrated detection of antibodies against the entire Mycob...
ORGANISM(S): Homo sapiens 
2010-07-13 | E-GEOD-19433 | biostudies-arrayexpress
ProteomicsProfiling proteome-scale antibody responses to M. tuberculosis proteins in TB suspect's sera
Human infection with Mycobacterium tuberculosis results in a continuum of ill-defined, clinical manifestations with stable latent M. tuberculosis infection (LTBI) and severe active disease at the ends. Identifying different states of infection is of importance to tuberculosis (TB) control since ris...
ORGANISM(S): Homo sapiens 
2012-07-05 | E-GEOD-30722 | biostudies-arrayexpress
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