Sort   by:  
 Page size 
TranscriptomicsTranscription profiling of migratory vs sedentary brown trout (Salmo trutta) and wild vs hatchery Atlantic salmon (Salmo salar)
A total of 55 individuals were analysed: 15 migratory brown trout (Salmo trutta) individuals from the Redon river, 15 sedentary brown trout (S. trutta) individuals from the Redon river, 15 sedentary brown trout (S. trutta) individuals from the Chevenne river, and 10 Atlantic salmon (S. salar) indivi...
ORGANISM(S): Salmo salar 
2006-03-27 | E-MEXP-123 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the proteins co-purified with Ada2b-PB-FLAG-HA FLAG-affinity purified from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell lines expressing Ada2b (isoform B; NP_001027151.1) in the pRmHa3-CHA2FL2 (Ada2b-PBH2F2) were maintained at 1-2 e6 cells/mL in SFX medium (HyQ SFX-Insect). Cells stably transfected with empty vector served as negative controls. Protein complexes containi...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-11-20 | MSV000083153 | MassIVE
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from Saccharomyces cerevisiae by FLAG-affinity purification and Size Exclusion Chromatography
Yeast protein extractions and subcellular fractionation Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-03-20 | MSV000085126 | MassIVE
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from human
Mammalian protein extraction and Halo purification For Halo purification, HEK293T cells at 40% confluency in a 100 mm plate were transfected with 10ug of plasmid containing ORF of PDHA1-HA-Halo in 1X BBS with 125 mM CaCl2. Cells were harvested by scraping in PBS following PBS wash after 24 hours fro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-20 | MSV000085125 | MassIVE
UnknownNanopore native RNA sequencing of a human poly(A) transcriptome.
Not available
S-EPMC7768885 | biostudies-literature
ProteomicsMudPIT analyses of the proteins associated with Human SETD2 deletion mutants
N-terminally Halo-tagged deletions of human SETD2 were constructed such as SETD2 ABCN1, A, B, C, CN, and CC consisted of amino acids 1-1692, 1-503, 504-1403, 1404-2564, 1404-1963, and 1964-2564, respectively. Deletion mutants were expressed in HEK293T cells and affinity purified, each in biological ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-08 | MSV000087587 | MassIVE
ProteomicsHalo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E2-DSSO-XL
Halo-SPIN1 SNAP-SPINDOC Seriel Capture Affinity Purified E2; on-beads DSSO crosslinking ms1/ms2/ms3 PD export (combined)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-18 | MSV000084719 | MassIVE
ProteomicsHalo-SPIN1_stable-cell-line_Halo-AP-MudPIT
Halo-SPIN1 stable cell line clone #9 & #11 in 293FRT cells Blank 293FRT control Halo purification Lys-C;Trypsin LTQ MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084679 | MassIVE
ProteomicsHalo-C11orf84_transient-transfection_Halo-AP-MudPIT
Transient transfection 293FRT cells Halo-C11orf84 and Halo-SNAP control Halo Purification Lys-C;Trypsine LTQ-MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-11 | MSV000084678 | MassIVE
ProteomicsHalo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E1/UB2/E2-MudPIT
Halo-SPIN1 SNAP-SPINDOC Seriel capture affinity purified E1;UB2;E2 MudPIT
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-17 | MSV000084713 | MassIVE
Sort   by:  
 Page size