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ProteomicsBeta-cell Galphas signaling is critical for physiological and pharmacological enhancement of insulin secretion
Islet lysates were adjusted to 5 percent SDS in TEAB, pH 8.5, reduced and alkylated and digested with modified trypsin (Worthington) using an S-trap mini device (Protifi). Peptides were labeled with TMT16 reagents (lot ZH350094). Phosphopeptide enrichment used GL Sciences p10 TiO tips. LC-MS/MS used...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2025-05-13 | MSV000097882 | MassIVE
GenomicsNatural genetic variation perturbs collaborative transcription factor binding required for enhancer selection and function
Natural genetic variation between two mouse strains was used as a natural mutagenesis screen to test various aspects of the proposed model for enhancer selection and function. Chromatin marks H3K4me2, H3K27Ac, and transcription factors PU.1, C/EBPa, and p65 were assessed by ChIP-Seq and GRO-Seq and ...
ORGANISM(S): Mus musculus 
2013-10-15 | E-GEOD-46494 | biostudies-arrayexpress
TranscriptomicsGenome-wide gene expression analysis during endotoxin tolerance in PBMCs.
Genome-wide gene expression was measured in peripheral blood mononuclear cells (PBMC) from healthy humans donors, four hours post stimulation of a single or double treatment of LPS and compared to untreated control PBMCs. We show that a second treatment with LPS induces endotoxin tolerance, with a ...
ORGANISM(S): Homo sapiens 
2011-05-17 | E-GEOD-22248 | biostudies-arrayexpress
GenomicsPS
Psoriasis cases as part of WTCCC2 phase 2
EGAD00010000124 | EGA
GenomicsGenome wide association scan in psoriasis
To identify novel susceptibility loci for psoriasis, we undertook a genome-wide association scan of of 594,224 SNPs in 2,622 individuals with psoriasis and 5,667 controls.
EGAS00000000108 | EGA
ProteomicsReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC
We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purifica...
ORGANISM(S): Mus musculus (Mouse) 
2014-09-22 | PXD000892 | Pride
Phosphoproteomics automation 
TranscriptomicsTranscriptome profiling of dedifferentiating smooth muscle cells from mouse aorta and bladder
Smooth muscle cells (SMCs) are important in a number of physiological systems and organs, including the cardiovascular system. The hallmark property of differentiated SMCs is the ability to contract, but contractile SMCs themselves show a range of phenotypes allowing prolonged tonic contraction in v...
ORGANISM(S): Mus musculus 
2016-07-01 | E-MTAB-4841 | biostudies-arrayexpress
ProteomicsPhosphoproteomic Response To MEK Inhibition
SILAC Heavy and Light cells were treated for 24 hours with each MEKi vs. Control in duplicate (Heavy/Light as well as Light/Heavy). For phosphoproteomic analysis, cells were treated for either 30 minutes or 24 hours with each MEKi vs. Control.
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD002621 | Pride
Mek phosphoproteomics tmt ape silac 
TranscriptomicsExpression analysis of a full SlrV locus 1 knockout in Haemophilus influenzae strain PittII
Investigation of whole genome gene expression differences between a full (four-gene) SlrV locus 1 deletion compared to the WT PittII strain. Removal of the four SlrV genes (aka macrophage survival factors : ?msf) results in a competetive defect with the WT strain in in vitro biofilms and in the oti...
ORGANISM(S): Haemophilus influenzae PittII 
2015-06-23 | E-GEOD-70172 | biostudies-arrayexpress
TranscriptomicsDeciphering the molecular immune response to COVID-19 using single cell multi-omics
Multi-omics single-cell profiling of surface proteins, gene expression and lymphocyte immune receptors from hospitalised COVID-19 patient peripheral blood immune cells and healthy controls donors. Identification of the coordinated immune cell compositional and state changes in response to SARS-CoV-2...
ORGANISM(S): Homo sapiens 
2021-03-17 | E-MTAB-10026 | biostudies-arrayexpress
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