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ProteomicsComputational prediction combined with proteogenomics unveils widespread non-AUG translation initiation events in plants
Non-AUG translation initiation is crucial for regulating translation, which can generate N-terminally extended protein isoforms with diverse biological functions. However, a comprehensive view of non-AUG translation initiation events with N-terminal extensions (NTEs) across monocots and dicots remai...
ORGANISM(S): Glycine max Zea mays (Maize) Oryza sativa (Rice) 
2026-04-27 | PXD058528 | Pride
Non-aug translation initiation  n-terminally extended protein isoforms  n-terminal extensions (ntes)  upstream translation initiation sites (utiss)  proteogenomics  monocots and dicots 
ProteomicsQuantitative analysis of changes in the phosphoproteome of maize induced by the plant hormone salicylic acid
Phytohormone salicylic acid (SA) plays an important role in regulating various physiological and biochemical processes. Our previous study identified several protein kinases responsive to SA, suggesting an important role for phosphorylation events in the plant response to SA.
ORGANISM(S): Zea mays (Maize) 
2018-10-24 | PXD002586 | Pride
Salicylic acid (sa) Maize Cellular processes Phosphoproteomic Itraq 
ProteomicsLarge-scale discovery of non-canonical peptides in monocot and dicot plants through an integrated peptidogenomic pipelineLarge-scale discovery of non-canonical peptides in monocot and dicot plants through an integrated peptidogenomic pipeline
Non-canonical peptides (NCPs) have been shown to play critical roles in fundamental biological processes. Despite their importance, NCPs have been rarely identified and characterized in plants. Here we developed an integrated peptidogenomic pipeline using high-throughput mass spectra to probe custom...
ORGANISM(S): Zea mays (Maize) 
2020-08-11 | PXD017081 | Pride
Plants Six-frame translation Mass spectrometry Peptidogenomics Non-canonical peptides 
ProteomicsLarge-scale discovery of non-canonical peptides in monocot and dicot plants through an integrated peptidogenomic pipeline
Non-canonical peptides (NCPs) have been shown to play critical roles in fundamental biological processes. Despite their importance, NCPs have been rarely identified and characterized in plants. Here we developed an integrated peptidogenomic pipeline using high-throughput mass spectra to probe custom...
ORGANISM(S): Zea mays (Maize) 
2020-08-11 | PXD017080 | Pride
Plant peptide lc-ms/ms 
ProteomicsDevelopmental dynamics of endogenous peptides in maize
In this study, we present a comprehensive peptidomic atlas of 13 tissues in maize spanning the vegetative and reproductive phases. Using 3-frame translation of canonical coding sequences (CDSs), we identified 6,100 non-redundant endogenous peptides, significantly expanding the known repertoire of pe...
ORGANISM(S): Zea mays (Maize) 
2025-05-07 | PXD055863 | Pride
Peptides Maize Lc-ms/ms 
ProteomicsComparative proteomic analysis of susceptible and resistance maize lines to southern corn rust
Southern corn rust (SCR) is one of destructive diseases in maize caused by Puccinia polysora Undrew. (P. polysara), widely occurring in warm-temperate and tropical regions globally. To identify candidate SCR resistance-related proteins and understand the molecular mechanism underlaying the maize and...
ORGANISM(S): Zea mays (Maize) 
2019-04-18 | PXD008904 | Pride
Southern corn rust Maize Proteomic 
ProteomicsReadthrough Events in Plants Reveal Unprecedented Plasticity of Stop Codons
Stop codon readthrough (SCR) has important biological implications but remains largely uncharacterized. Here, we identify 1,009 SCR events in plants using a proteogenomic strategy. Plant SCR candidates tend to have shorter transcript lengths and fewer exons and splice variants than non-SCR transcrip...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Glycine max Zea mays (Maize) Oryza sativa (Rice) 
2024-02-02 | PXD035958 | Pride
Proteogenomics Mass spectrometry Stop codon readthrough Recoding plasticity Amino acids Plants 
ProteomicsReadthrough Events in Plants Reveal Unprecedented Plasticity of Stop Codons
Stop codon readthrough (SCR) has important biological implications but remains largely uncharacterized. Here, we identify 1,009 SCR events in plants using a proteogenomic strategy. Plant SCR candidates tend to have shorter transcript lengths and fewer exons and splice variants than non-SCR transcrip...
ORGANISM(S): Glycine max Arabidopsis thaliana (Mouse-ear cress) Zea mays (Maize) Oryza sativa (Rice) 
2024-02-02 | PXD038762 | Pride
Proteogenomics Mass spectrometry Stop codon readthrough Recoding plasticity Amino acids Plants 
ProteomicsNUT1-Exo70A1 Regulates Xylem Vessel Development and Influences Water Use Efficiency in Maize
The total proteins from transgenic plants overexpressing Exo70A1 coding sequence in a translational fusion of a GFP-tag were extracted from root stele tissues using a lysis buffer composed of 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA pH 8.0, 0.5% NP-40, and freshly added 1 mM PMSF, 1 mM DTT, 1...
ORGANISM(S): Zea Mays 
2026-01-20 | PXD073273 |
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