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ProteomicsComparison of affinity enrichment methods for O-GlcNAc proteomics
In this work, by using the lysates of PANC-1 (a pancreatic cancer cell line), we provided a head-to-head comparison of three affinity enrichment methods/materials (i.e., antibody, lectin AANL6, and an OGA mutant) for O-GlcNAc proteomics. The enrichment was performed before or after high-pH HPLC frac...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2024-04-23 | MSV000094604 | MassIVE
ProteomicsOptimization of O-GIG for O-glycopeptide Characterization with Sialic Acid Linkage Determination-Fig6
O-glycoprotein analysis has been historically challenging due, in part, to a dearth of available enzymes active in release of O-glycans. Moreover, chemical releasing methods, such as β-elimination/Michael addition are not specific to O-glycan release and can also eliminate phosphoryl substitutions....
ORGANISM(S): Bos taurus (Bovine) 
2020-07-28 | PXD020034 | Pride
Mucin O-glycopeptide immobilization Operator Lactoferrin O-glycosylation Sialic acid 
ProteomicsOptimization of O-GIG for O-glycopeptide Characterization with Sialic Acid Linkage Determination-Fig3
O-glycoprotein analysis has been historically challenging due, in part, to a dearth of available enzymes active in release of O-glycans. Moreover, chemical releasing methods, such as β-elimination/Michael addition are not specific to O-glycan release and can also eliminate phosphoryl substitutions....
ORGANISM(S): Bos taurus (Bovine) 
2020-07-28 | PXD020048 | Pride
Mucin O-glycopeptide immobilization Operator Lactoferrin O-glycosylation Sialic acid 
ProteomicsOptimization of O-GIG for O-glycopeptide Characterization with Sialic Acid Linkage Determination -Figure 2
O-glycoprotein analysis has been historically challenging due, in part, to a dearth of available enzymes active in release of O-glycans. Moreover, chemical releasing methods, such as β-elimination/Michael addition are not specific to O-glycan release and can also eliminate phosphoryl substitutions....
ORGANISM(S): Bos taurus (Bovine) 
2020-07-28 | PXD020039 | Pride
Mucin O-glycopeptide immobilization Operator Lactoferrin O-glycosylation Sialic acid 
ProteomicsOptimization of O-GIG for O-glycopeptide Characterization with Sialic Acid Linkage Determination-figure 5
O-glycoprotein analysis has been historically challenging due, in part, to a dearth of available enzymes active in release of O-glycans. Moreover, chemical releasing methods, such as β-elimination/Michael addition are not specific to O-glycan release and can also eliminate phosphoryl substitutions....
ORGANISM(S): Bos taurus (Bovine) 
2020-07-28 | PXD020032 | Pride
Mucin O-glycopeptide immobilization Operator Lactoferrin O-glycosylation Sialic acid 
ProteomicsOptimization of O-GIG for O-glycopeptide Characterization with Sialic Acid Linkage Determination-Figure 4
O-glycoprotein analysis has been historically challenging due, in part, to a dearth of available enzymes active in release of O-glycans. Moreover, chemical releasing methods, such as β-elimination/Michael addition are not specific to O-glycan release and can also eliminate phosphoryl substitutions....
ORGANISM(S): Bos taurus (Bovine) 
2020-07-28 | PXD020053 | Pride
Mucin O-glycopeptide immobilization Operator Lactoferrin O-glycosylation Sialic acid 
ProteomicsSystematic Quantification of Protein O-GlcNAcylation Reveals Common and Cell-Type-Specific Responses to N-Glycosylation Inhibition in Human Cells
In this work, we systematically and site-specifically analyzed protein O-GlcNAcylation under the N-glycosylation inhibition through the integration of metabolic labeling, bio-orthogonal chemistry, and multiplexed proteomics. The experiments were performed in three types of human cells, i.e., HEK293T...
ORGANISM(S): Homo sapiens (Human) 
2026-06-02 | PXD073249 | Pride
Glycoproteomics Protein o-glcnacylation 
ProteomicsThe HLA ligandome comprises a limited repertoire of O-GlcNAcylated antigens preferentially associated with HLA-B07:02
Mass-spectrometry based immunopeptidomics has provided unprecedented insights into antigen presentation, not only charting an enormous ligandome of self-antigens, but also cancer neo-antigens and peptide antigens harbouring post-translational modifications. Here we concentrate on the latter, focusin...
ORGANISM(S): Homo sapiens (Human) 
2021-12-03 | PXD028874 | Pride
O-glcnacylation Hla-b07 Mhc Hla class i Immunopeptidomics 
ProteomicsDifferentially O-GlcNAcylated proteins and sites in Y.pestis
Differentially O-GlcNAcylated proteins and sites in Y. pestis strains under Fv and Mh.
ORGANISM(S): Yersinia Pestis Co92 
2023-06-12 | PXD042927 |
ProteomicsImmunoprecipitation of YAP - The essential role of YAP O-GlcNAcylation in high-glucose-stimulated liver tumorigenesis
YAP was immunoprecipitated to detect O-GlcNAcylation status.
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD005992 | Pride
Liver yap o-glcnacylation 
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