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ProteomicsSystematic and Quantitative Investigation of Newly Synthesized Proteins Reveals Distinct Ion Homeostasis and Mitochondrial Changes between Cuproptosis and Ferroptosis in Human Cells
To better understand ferroptosis and cuproptosis, here we systematically and quantitatively analyzed newly synthesized proteins (NSPs), which reflect rapid changes in gene expression during cell death without the interference from the existing proteins. Integrating metabolic labeling with multiplexe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-11-13 | MSV000099889 | MassIVE
ProteomicsA model considering the different forms of a protein to accurately describe protein degradation in human cells
Searching parameter: 10 ppm precursor mass tolerance; 0.025 Da product ion mass tolerance; fully digested with trypsin; up to two missed cleavages; Variable modifications: oxidation of methionine (M+15.9949); heavy lysine (K+8.0142); heavy arginine (R+6.0201); Fixed modifications: carbamidomethylati...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-20 | MSV000086337 | MassIVE
ProteomicsSystematic evaluation of protein synthesis inhibition through global quantification of newly synthesized protiens
Manipulating protein synthesis is commonly applied in bio-related research to uncover protein functions and cellular activities. Multiple inhibitors with distinct mechanisms are widely used, but it is extraordinarily challenging to systematically determine and evaluate their inhibition efficiencies,...
ORGANISM(S): Homo sapiens (Human) 
2023-05-24 | PXD035992 | Pride
Bioorhogonal chemistry  protein synthesis inhibtion  protein inhibition efficiency  mass spectromety  multiplexed proteomics  newly synthesized proteins 
ProteomicsInvestigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling
Heat shock proteins are responsible for protein folding in cells. HSP90 is one of the most important chaperones in human cells, and inhibiting HSP90 is a potential strategy for cancer therapy. Multiple HSP90 inhibitors have been in clinical trials. However, none of them has been approved for disease...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037575 | Pride
Heat shock protein 90 Hsp90 inhibitors And protein abundance change Thermal proteome profiling Cell response Protein thermal stability shift 
ProteomicsQuantitative structural proteomics unveils the conformational changes of proteins under ER stress
Using cysteine footprinting to globally profile protein structure changes. The method was further applied to study the unfolding effect of tunicamycin.
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-04-25 | PXD028864 | Pride
Human hek293t lc-ms/ms 
ProteomicsSystematic Quantification of Protein O-GlcNAcylation Reveals Common and Cell-Type-Specific Responses to N-Glycosylation Inhibition in Human Cells
In this work, we systematically and site-specifically analyzed protein O-GlcNAcylation under the N-glycosylation inhibition through the integration of metabolic labeling, bio-orthogonal chemistry, and multiplexed proteomics. The experiments were performed in three types of human cells, i.e., HEK293T...
ORGANISM(S): Homo sapiens (Human) 
2026-06-02 | PXD073249 | Pride
Glycoproteomics Protein o-glcnacylation 
ProteomicsEthanol Exposure during Differentiation of Human Induced Pluripotent Stem Cells Reduces Cardiomyocyte Generation and Alters Metabolism
In this study, we investigated the effects of ethanol exposure on cardiomyocyte differentiation by treating the cells with various concentrations of ethanol. The results indicated that ethanol exposure during hiPSC-CM differentiation reduced cell viability, cell proliferation, and cardiomyocyte yiel...
ORGANISM(S): Homo sapiens (Human) 
2025-05-23 | PXD056092 | Pride
Cardiomyocyte differentiation Ethanol treatment 
ProteomicsA chemoproteomics method for direct and comprehensive characterization of cysteine reversible oxidation
Cysteine oxidation is a series of protein modifications caused by reactive oxygen species (ROS) in cells and can regulate protein functions and cellular activities. Comprehensive and site-specific characterization of the cysteine oxidation is critical for understanding the role of ROS regulating cel...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-20 | MSV000093703 | MassIVE
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