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ProteomicsQuantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production
Cytosolic lipid droplets (LDs) are vital to Hepatitis C Virus (HCV) infection as the putative sites of virion assembly. To identify novel regulators of HCV particle production, we performed quantitative LD proteome analysis. Huh7.5 cells were labeled by stable isotope labeling with heavy amino acids...
ORGANISM(S): Homo sapiens (Human) 
2016-09-26 | PXD004707 | Pride
Lc-msms Hcv Silac Quantitative proteomics Lipid droplets 
ProteomicsHomogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization
Tissue samples from human tonsils and rat pancreas were subjected to a novel laser-based homogenization method, the PIRL-DIVE homogenization, and to a conventional mechanical tissue homogenization procedure (bead mill and a cryo-grinder). The protein species composition of the protein extracts were ...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2016-01-25 | PXD003434 | Pride
Protein species Sample preparation Proteolysis Pirl Tissue homogenization Lc-msms Pirl-dive 
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