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ProteomicsOptimization of a Sample Preparation Workflow for Proteomics Analysis of the Mouse Feces
We evaluated different protein different extraction protocols (using different lysis buffers) and contaminant removal strategies during bottom-up proteomics analysis of mouse feces to maximize quantitative reproducibility and the number of identified proteins.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-22 | PXD027788 | Pride
Filtration method Sample preparation Optimization Bottom-up proteomics Lysis buffer 
ProteomicsNative mass spectrometry of an allosteric heterodimer of SARS-CoV-2
The capability of native MS to probe protein-protein and protein-ligand interactions was exemplified with the allosteric heterodimer from SARS-CoV-2 consisting of the nonstructural proteins (nsp) nsp10 and nsp16. Complex dynamics, allostery, and ligand binding were shown. A metadata Excel file, 'nsp...
ORGANISM(S): Escherichia Coli Bl21(de3) (ncbitaxon:469008) 
2023-08-28 | MSV000092776 | MassIVE
ProteomicsStructure elucidation for tunable hetero-assembly of a plant PDX1.2/PDX1.3 pseudoenzyme-enzyme pair
Mass spectrometry raw data including peptide mapping, and top down liquid chromatography mass spectrometry for Arabidopsis PDX1.2/PDX1.3 homo- and hetero-complexes produced using cell-free expression. Purified proteins from cell-free expression were digested using trifluoroethanol protocol for pepti...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-04-06 | MSV000085233 | MassIVE
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