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ProteomicsIdentification of ERK1 direct substrates using stable isotope labeled kinase assay linked phosphoproteomics
Following the kinase assay linked phosphoproteomics strategy (KALIP) (Xue, L et al., 2012), we used extracellular signal-regulated kinases 1 (ERK1) to phosphorylate the HEK293 cell lysate under the in vitro kinase assay condition. The phosphorylated proteins were then isolated and identified by mass...
ORGANISM(S): Homo sapiens (Human) 
2014-07-17 | PXD000357 | Pride
Erk1 Phosphoproteomics Kinase substrates 
TranscriptomicsExpression data from murine colon tissue after exposure to 4% DSS for 6 days DSS followed by 4 days of water or 1% L-arginine
L-Arginine (L-Arg) is the substrate for both inducible nitric oxide synthase and arginase, which are upregulated in human IBD and in mouse colitis models. We have found that L-Arg supplementation enhances wound restitution in vitro, and improves the clinical parameters of weight loss, survival, and ...
ORGANISM(S): Mus musculus 
2012-06-26 | E-GEOD-34874 | biostudies-arrayexpress
MetabolomicsA Combined Proteomic and Metabolomic Strategy for Allergens Characterization in Natural and Fermented Brassica napus Bee Pollen

Bee pollen is consumed for its nutritional and pharmacological benefits, but it also contains hazardous allergens which has not been identified. Here, we identified 2 potential allergens, glutaredoxin and oleosin-B2, in Brassica napus (B. napus) bee pollen using mass spectrometr...

2022-03-23 | MTBLS3950 | MetaboLights
TranscriptomicsExpression data from rice embryo and endosperm development
The receptor-like kinases (RLKs) plays critical roles in signal transduction through sensing the extracellular signals and activating the downstream signal transduction by phosphorylating their targets. Up to now, there are only a few RLKs have been functionally identified. In this study, we systemi...
ORGANISM(S): Oryza sativa 
2012-02-01 | E-GEOD-27856 | biostudies-arrayexpress
TranscriptomicsGene expression profile in yeast under physiological changes of stress adaptation and stress recovery
The data provide information expression profile in yeast for 5 different physioloigcal conditions during stress adpatation and stress recovery (normal growth, during stress adaptation, after stress adaptation, under stress recovery, after stress recovery) in yeast. The purpose of the study is to u...
ORGANISM(S): Saccharomyces cerevisiae 
2013-07-22 | E-GEOD-36599 | biostudies-arrayexpress
ProteomicsCardiomyocyte OTUD1 drives diabetic cardiomyopathy via directly deubiquitinating AMPKα2 to inhibit AMPK activity and induce mitochondrial dysfunctionCardiomyocyte OTUD1 drives diabetic cardiomyopathy via directly deubiquitinating AMPKα2 to inhibit AMPK activity and induce mitochondrial dysfunction
The sample was removed from-80℃ and melted on ice. An appropriate amount of TEAB was added to adjust pH 8.0.5 μ L of suspension was used for SDS-PAGE testing.
ORGANISM(S): Homo sapiens (Human) 
2025-06-11 | PXD061479 | Pride
Human，cells，lc-msms 
UnknownDiterpenoids with Potent Anti-Psoriasis Activity from Euphorbia helioscopia L.
Not available
S-EPMC11397010 | biostudies-literature
MetabolomicsComparative metabolomic analysis of exudates of microcystin-producing and microcystin-free Microcystis aeruginosa strains

Cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis aeruginosa threaten the ecological integrity and beneficial uses of lakes globally. In addition to producing hepatotoxic microcystins (MC), M. aeruginosa exudates (MaE) contain various compounds with demonstrat...

2023-01-16 | MTBLS6603 | MetaboLights
UnknownAntihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats.
Not available
S-EPMC9367739 | biostudies-literature
ProteomicsVCP–adaptor interactions are exceptionally dynamic and subject to differential modulation by a VCP inhibitor
In this study we use a combination of mass spectrometry-based proteomics and biophysical studies to characterize the interaction of adaptors with VCP. Our results reveal that most VCP–adaptor interactions are characterized by exceptionally rapid dynamics that in some cases are modulated by the VCP i...
ORGANISM(S): Homo sapiens (Human) 
2016-07-18 | PXD004105 | Pride
Ap-ms Sec-ms Vcp/p97 Protein complex dynamics Crosslinking-ms Orbitrap 
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