Photosynthesis relies on a dynamic network of protein-protein interactions that adapt to fluctuating light conditions. We present an XL-MS pipeline applied directly to functional thylakoid membranes from Arabidopsis thaliana and Spinacia oleracea. By combining PhoX crosslinker with the amphiphilic s...
Cells are comprised of a broad spectrum of structures that compartmentalize biochemical and signaling mechanisms. These structures can be comprised of many biomolecules, but especially lipids, proteins, and nucleic acids. Options are limited to quantify or discover new subcellular structures. We exp...
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
interrogate the 3D organization of the MCM2-7 DH-Sld3/7 complex espetically the flexible Sld3/7 regions, the MCM2-7 DH-Sld3/7 complex was analyzed by crosslinking MS
Our BATCH assay suggested that glycolytic enzymes could associate into complexes of varied stoichiometry and potentially interact with proteins outside of glycolysis, moreover, PfkA, FbaA and GapA were the top interacting proteins, showing strong interactions with both the PTS and PNPase. We constru...
