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Transcriptional profiling of S. mutans rpoE knockout mutant comparing to the wild type. The mutation of rpoE cause dramatic changes in phenotypes. The goal is to determine the genes affected by rpoE. The delta subunit of RNA polymerase, RpoE, is wide spread in low G+C Gram-positive bacteria and is t...
ORGANISM(S): Streptococcus mutans UA159 
The genomic DNA from exponential phase cells was cross-linked and digested with 200 unit MboI enzyme (NEB) as previously described36. The cutting DNA ends were labelled with biotin-14-dCTP (TriLINK) followed by ligation. Purified DNA was sheared to a length of ~400 bp. Point ligation junctions were ...
2018-12-26 | OEX00000016 | NODE
The BY4742 and SY14 cells from early-log phase (OD600 = 1) were collected, and their RNA was isolated using the TRIzol (Invitrogen) method45. The library preparation followed the standard procedure (BGI). The libraries were sequenced on the Illumina HiSeq 4000 platform using the 150-bp pair-end sequ...
2018-12-26 | OEX00000017 | NODE
Ticks are widely distributed ectoparasitic arthropods that suck blood from the body surface of livestock, wild animals, and humans. Ticks not only transmit a variety of pathogens but also cause various degrees of damage to their hosts' skin during blood feeding. To explore the molecular regulatory m...
ORGANISM(S): Haemaphysalis Longicornis 
2020-02-24 | PXD017694 |
Proteomic of Cardamine violifolia treated by sodium selenate
ORGANISM(S): Cardamine Circaeoides 
2019-12-02 | PXD016541 |
The difficulty to obtain data from whom uninterruptedly practice meditation over many years, and lacking of advanced analysis methods significantly hinder the comprehensive understanding of molecular mechanisms underlying meditation. This study aimed to address this issue.Accordingly, we recruited 8...
ORGANISM(S): Homo Sapiens 
2018-09-27 | PXD057418 |
Mice were sacrificed after anesthesia by isoflurane following neck cutting. The spinal cord L3-L5 was taken and frozen in liquid nitrogen immediately and the ventral horn of the bilateral spinal cord (about 400um thick in gray matter area) was collected at low temperature. The samples were digested ...
ORGANISM(S): Mus Musculus 
2023-06-12 | PXD042929 |
To outline the temporal changes of long COVID, we profiled serum proteomes (dataset SP2) and urine proteomes (dataset UP2). The SP2 were measured for 37 patients during the disease (T1), the convalescence (T2), and at the 1-year revisit (T3); the UP2 were measured for 28 patients during the disease ...
ORGANISM(S): Homo Sapiens 
2024-06-04 | PXD027557 |
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