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Irgb6-deficient MEFs stably expressing Spot-tagged Irgb6 were infected with or without T. gondii ME49 for 4 h and lysed in guanidine buffer (6 M guanidine-HCl, 100 mM Tris-HCl, pH 8.0, and 2 mM DTT). The lysates were diluted 8-fold with RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM EGTA, ...
ORGANISM(S): Mus Musculus (mouse) 
MM1.S cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and ide...
ORGANISM(S): Homo Sapiens (human) 
HEK293T cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide, GA-TD-3 or GA-TD-5 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and i...
ORGANISM(S): Homo Sapiens (human) 
HCC827-Luc cells stably expressing VHL-AirID were pretreated with MG132. The DMSO, VH032 or MS39 were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV ...
ORGANISM(S): Homo Sapiens (human) 
HCC827-Luc cells stably expressing ThBD-AGIA-AirID were pretreated with MG132. The DMSO, Pomalidomide or MS154 were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Ta...
ORGANISM(S): Homo Sapiens (human) 
MM1.S cells stably expressing ThBD-AGIA-AirID were treated with DMSO, Pomalidomide or ARV-825 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified b...
ORGANISM(S): Homo Sapiens (human) 
MM1.S cells stably expressing VHL-AirID were treated with DMSO, VH032 or MZ1 with MG132 in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analys...
ORGANISM(S): Homo Sapiens (human) 
LNCaP cells stably expressing ThBD-AGIA-AirID were pretreated with MG132. Pomalidomide or ARV-110 was added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV ...
ORGANISM(S): Homo Sapiens (human) 
NCI-H226 cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identifi...
ORGANISM(S): Homo Sapiens (human) 
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