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Data-independent acquisition (DIA) techniques, such as SWATH acquisition, have emerged as the preferred strategy for proteomic analysis. Our study optimizes the SWATH-DIA method using a narrow isolation window placement approach improving its proteomic performance. We optimized the acquisition param...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms Mus Musculus (mouse) 
In the present study, we examined duodenal gene expression in rats subjected to immobilization, to elucidate the mechanism of the stress response in the duodenum. Experiment Overall Design: Total RNA was extracted from the duodenal mucosa with an acid guanidinium thiocyanate-phenol-chloroform mixtur...
ORGANISM(S): Rattus norvegicus 
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
Nrf2 (NF-E2-related factor-2) transcription factor regulates oxidative/xenobiotic stress response and also represses inflammation. However, the mechanisms how Nrf2 alleviates inflammation are still unclear. Here, we demonstrate that Nrf2 interferes with lipopolysaccharide-induced transcriptional upr...
ORGANISM(S): Mus musculus 
Proteome analysis of reeled spider silk and web in captive environments
ORGANISM(S): Cyclosa Laticauda Argiope Amoena Yaginumia Sia Dictyna Felis Cyclosa Octotuberculata Parasteatoda Tepidariorum Badumna Insignis Argiope Bruennichi Lycosa Suzukii Trichonephila Clavata Tetragnatha Praedonia 
We performed a DIA-MS proteomic analysis of sera from systemic juvenile idiopathic arthritis with different activity phases using a high protein depletion process. We profiled the proteins in the sera that differed significantly in their activity phase.
ORGANISM(S): Homo Sapiens (human) 
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