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ProteomicsHEK-293T LC-MS/MS
We established two in vitro cell-free systems to mimic the activation of mTORC1 and autophagy by monitoring the translocation of two readout proteins Raptor and ATG14. Based on the two systems, we further screened the potential regulators involved in the leucine-mediated mTORC1 and autophagy pathway...
ORGANISM(S): Homo sapiens (Human) 
2022-03-03 | PXD007811 | Pride
Human Cell-free system Lc-ms/ms 
ProteomicsHEK293T LC-MS/MS
Leucine plays crucial roles in protein proteolytic autophagy process, but the underlying mechanisms are not fully understood. Here, we combined in vitro reconstitution with comparative proteomics technology to identify the potential regulators for leucine-mediated autophagy pathway. We established a...
ORGANISM(S): Homo sapiens (Human) 
2022-03-02 | PXD007845 | Pride
Hek293t Cell-free system Leucine Itraq 
ProteomicsProteomic analysis of glutamine deprivation in HepG2 cells
We applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. Two independent cultured cell lysates, namely control and glutamine deprivation, were marked with iTRAQ labels and subjected to LC-ESI-MS/MS analysis. The t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080721 | MassIVE
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