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In this study, we performed the first dynamic proteome analysis of wheat seed germination using a two-dimensional differential gel electrophoresis (2D-DIGE)-based proteomic approach. A total of 166 differentially expressed protein (DEP) spots representing 73 unique proteins were identified.
ORGANISM(S): Triticum aestivum (Wheat) 
2015-12-09 | PXD003083 | Pride
Using high accuracy nano LC-MS/MS combined with affinity purification, we identified 636 lysine acetylated peptides matched on 353 proteins, and these acetylated proteins were implicated in various molecular functions and basic cellular processes by gene ontology (GO), Kyoto Encyclopedia of Genes an...
ORGANISM(S): Brachypodium distachyon (Purple false brome) (Trachynia distachya) 
2018-10-25 | PXD003161 | Pride
Protein lysine succinylation, an emerging protein post-translational modification widespread among microbiology, animals and plants, represents an important regulator of cellular processes. In our study, we took the first succinylation proteome analysis in the seedling leaves of Bd21 to draw a schem...
ORGANISM(S): Brachypodium distachyon (Purple false brome) (Trachynia distachya) 
2018-10-26 | PXD003428 | Pride
Waxy starch has an important influence on bread dough and the qualities of breads. Generally, grain weight and yield in waxy wheat (Triticum aestivum L.) are significantly lower than in bread wheat. In this study, we performed the first proteomic and phosphoproteomic analyses of starch granule-bindi...
ORGANISM(S): Triticum aestivum (Wheat) 
2017-10-30 | PXD002805 | Pride
Exercise induces skeletal muscle adaptation, and the p38 mitogen-activated protein kinase signaling pathway is thought to play an important role in the adaptive processes. We have obtained new evidence that the gamma isoform of p38 is required for exercise-induced metabolic adaptation in skeletal mu...
ORGANISM(S): Mus musculus 
The floral transition in Arabidopsis is tightly controlled by complex genetic regulatory networks in response to endogenous and environmental flowering signals. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and SHORT VEGETATIVE PHASE (SVP), two key MADS-domain transcription factors, perceive the...
ORGANISM(S): Arabidopsis thaliana 
Drought stress is a major abiotic stress affecting plant growth and development. Brachypodiumdistachyon L. is a special model plant for wheat, barley, and several potential biofuel grasses. In this study, we make comparative phosphoproteome analysis of seeding leaves at the three leaf stage in Brach...
ORGANISM(S): Brachypodium distachyon (Purple false brome) (Trachynia distachya) 
2018-10-26 | PXD003522 | Pride
In this study, we carried out the first large-scale phosphoproteome analysis of seedling leaves in Brachypodium accession Bd21 using TiO2 microcolumns combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MaxQuant software. A total, 1,470 phosphorylation sites in 950 phosphopro...
ORGANISM(S): Brachypodium distachyon (Purple false brome) (Trachynia distachya) 
2016-07-09 | PXD000868 | Pride
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