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TranscriptomicsLong Noncoding RNA HNF1A-AS1 Regulates Proliferation and Migration in Esophageal Adenocarcinoma Cells
Objectives: Long non-coding RNAs (lncRNAs) have been shown to play important roles in the development and progression of cancer. However, functional lncRNAs and their downstream mechanisms are largely unknown in the molecular pathogenesis of esophageal adenocarcinoma (EAC) and its progression. Desig...
ORGANISM(S): Homo sapiens 
2013-10-21 | E-GEOD-48240 | biostudies-arrayexpress
ProteomicsCold-induced phosphorylational sites analysis of protein kinase OST1
For the analysis of phosphorylation sites of OST1, 8 g pSuper:OST1-Myc plants materials, treated with 4oC for 0 or 2 h, were used for protein extraction and immunoprecipitated with anti-Myc agarose. Then OST1-Myc proteins were eluted with 0.1 M glycine solution (pH 2.8) and mixed with 1 M Tris solut...
ORGANISM(S): Arabidopsis Thaliana 
2020-11-16 | PXD022539 |
ProteomicsAnalysis of phosphorylation sites of ZmRR1 phosphorylated by ZmMAPK8
To identify the phosphorylation site of ZmRR1 by ZmMPK8 in vitro, 10 µg GST-ZmRR1 and 1 µg His-ZmMPK8 purified proteins were incubated in 20 µl of protein kinase reaction buffer containing 20 mM MgCl2, 50 mM Tris-HCl pH 7.5, 1 mM DTT, and 50 µM ATP, at 30°C for 30 min. The reaction products were red...
ORGANISM(S): Zea Mays 
2021-07-16 | PXD027378 |
ProteomicsIdentification of ANXA1 as A Novel Upstream Negative Regulator of Notch1 Function in AML
Abnormal Notch1 expression has an important role in tumorigenesis. However, upstream control mechanisms for Notch1 are still insufficiently understood. Acute myeloid leukemia (AML) is one of the most common and lethal blood malignancies with limited possibilities for treatment. Thus, new therapeutic...
ORGANISM(S): Homo Sapiens 
2024-10-03 | PXD056593 |
ProteomicsRNA-Pull down and MS for Snhg3
To screen Snhg3-binding proteins using-RNA Pull down and MS
ORGANISM(S): Mus Musculus 
2023-01-17 | PXD039526 |
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