For identification of Mi-2β-interacting proteins, B16F10 cells (1 × 108) were collected and washed three times with PBS and lysed in lysis buffer on ice for 30 min. Cell lysates were collected and then isolated over night by anti-Mi-2β magnetic agarose beads. The anti-Mi-2β magnetic agarose beads we...
This is the model described in the article:
A bistable Rb-E2F switch underlies the restriction point
Guang Yao, Tae Jun Lee, Seiichi Mori, Joseph R. Nevins, Lingchong You, Nat Cell Biol
2008 10:476-482; PMID: 18364697
; D...
We analyzed the lactylomes and proteomes of infarcted myocardium in mice subjected to sham treatment, myocardial infarction (MI) for 30 min or MI followed by I/R for 6 h using liquid chromatography-tandem mass spectrometry (LC–MS/MS) system.
Long non-coding RNAs (lncRNAs) are emerging regulators of genomic stability and human disease. However, the molecular mechanisms by which nuclear lncRNAs directly contribute to DNA damage responses remain largely unknown. Using RNA antisense purification coupled with quantitative mass spectrometry (...
To systematically identify interaction proteins and phophorylation modifications of CRTC2 in response to DNA damage, we performed PTM assessments by using unbiased, label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) with CRTC2 pulldown lysates from irradiation (IR)-treated HCCLM3 c...
Coukos R, Yao D, Sanchez MI, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY. 2021. The trafficking of specific protein cohorts to the correct subcellular location at the correct time is essential for every signaling and regulatory process in biology. Gene perturbation scree...
