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Gene regulation at the maternal-embryonic transition in the pre-implantation mouse embryo is not well understood. We knock down Ccna2 to establish proof-of-concept that antisense morpholino oligonucleotides can be used to target specific genes. We applied this strategy to study Oct4 and discovered...
ORGANISM(S): Mus musculus 
Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks; of age. 14 days after ovariectomy, mice were injected; subcutaneously with progesterone dissolved in sesame oil (Sigma;; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine; horns at various times after injection. 8 ...
ORGANISM(S): Mus musculus 
Female mice used were SvJ/129, 10-16 weeks of age; Hoxa-10+/+ and Hoxa-10-/- littermates were used in parallel experiments. 14 days after ovariectomy, mice were injected subcutaneously with progesterone dissolved in sesame oil (Sigma; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine ...
ORGANISM(S): Mus musculus 
Female mice used were 129/SvImJ wildtype (Jackson Lab), 10-16 weeks; of age. 14 days after ovariectomy, mice were injected; subcutaneously with progesterone dissolved in sesame oil (Sigma;; 0.2mg/0.1mL/mouse), followed by sacrifice and removal of uterine; horns at various times after injection. 8 ...
ORGANISM(S): Mus musculus 
Female mice used were SvJ/129, 10-16 weeks of age; Hoxa-10+/+ and Hoxa-10-/- littermates were used in parallel experiments. 14 days after ovariectomy, mice were injected with sesame oil; (Sigma; 0.1mL/mouse), followed by sacrifice and removal of uterine; horns at 6 hours after injection. 8 uterine...
ORGANISM(S): Mus musculus 
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