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ProteomicsIsotopic-labelled Clickable Glutathione to Quantify Protein S-Glutathionylation
Investigated protein glutathionylation in HL-1 cardiomyocyte cells in response to hydrogen peroxide using a clickable glutathione approach. After exposure, or not, to hydrogen peroxide the glutathionylated proteins with heavy or light azido glutathione were enriched and subjected to LC-MS/MS analysi...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD014937 | Pride
Clickable glutathione Glutathionylation Cardiomyocyte 
ProteomicsSite-specific Identification and Quantification of Protein Glutathionylation under Ischemic Stress
Ischemia or ischemic reperfusion is well-known for its contribution to heart diseases. During ischemia and reperfusion, a flux of nutrients and oxygen to cardiomyocytes is altered, which causes a burst of ROS from mitochondria and other enzymes. Elevated levels of ROS can cause oxidative modificatio...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-13 | PXD024766 | Pride
Gutathionylation Cardiomyocytes Click chemistry 
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