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The experiment investigates the consequences of Yjr141w downregulation on gene expression in yeast. The essential protein Yjr141w was depleted from cells using a tagged variant (Yjr141w-psd). The photo-sensitive degron (psd) module initiates degradation of the tag and its associated protein upon blu...
ORGANISM(S): Saccharomyces cerevisiae 
Cyanothece sp. ATCC 51142 12 hour light + 12 hour dark + 24 h light in a 48 hour time course
ORGANISM(S): Cyanothece sp. 
High-density kinetic analysis of the metabolomic and transcriptomic response of Arabidopsis to temperature and light
ORGANISM(S): Arabidopsis thaliana 
To determine the effect of RNA degradation on miRNA expression profiling. To mimic total RNA degradation in snap-frozen livers and duodenums from mice (wild type female, C57/Bl6) tissues were stored in a -80°C freezer and then transferred to dry ice. Each frozen tissue was sliced into five identica...
ORGANISM(S): Mus musculus 
In the yeast saccharoryces cerevisiae, RPA49 full-deletion mutants show a strong growth defect at 30°C and are unable to grow at 25°C. However, spontaneous suppressors that restore growth at 25°C are observed. We attend to identified new suppressor alleles after UV treatment. To mapped the genomic l...
ORGANISM(S): Saccharomyces cerevisiae 
The goal of this study was to compare the transcriptional profile (RNA-seq) of wild type plants with that of link1link2 double mutants grown under continous light or long day (16h light/8 h darkness) conditions. In the first experiment wild-type and link1link2 mutant plants were grown for two weeks ...
ORGANISM(S): Arabidopsis thaliana 
The field of optogenetics offers a powerful toolbox to control cellular processes. We used the photo activatable adenylate cyclase from Beggiatoa to restrict cell-cycle progression under specific light conditions. In a label-free proteomics experiment we investigated the influence of cell-cycle rest...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-04-24 | PXD046730 | Pride
Parallel germination of Cul3a-1 mutant and control plants in far red light.
ORGANISM(S): Arabidopsis thaliana 
In plants, reactive oxygen species and, more particularly, hydrogen peroxide (H2O2) play a dual role as toxic by-products of normal cell metabolism and as regulatory molecules in stress perception and signal transduction. Peroxisomal catalases are an important sink for photorespiratory H2O2. Using A...
ORGANISM(S): Arabidopsis thaliana 
Elimination of misfolded proteins is crucial for proteostasis and to prevent proteinopathies. Nedd4/Rsp5 emerged as a major E3 ligase involved in multiple quality control pathways that target misfolded plasma membrane proteins, aggregated polypeptides, and cytosolic heat-induced misfolded proteins f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-01 | PXD004747 | Pride
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