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TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in matrigel or a non-adhesive alginate hydrogel after 28 days of in vitro growth. Additionally, we used scRNA-seq to profile HIOs derived in the presence of Neureguli...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10268 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids, adult intestine, and human fetal endoderm derived organs
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human definitive endoderm and intestinal organoids (HIOs) at several timepoints of in vitro growth (7, 14, and 28 days) and after in vivo growth beneath the kidney capsule of a murine host (4 and 8 wks post...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10187 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal lung primary tissues and cell cultures derived from fetal bud tip progenitors under dual SMAD treatment
Underdeveloped lungs are a primary cause of morbidity and mortality in premature infants, but our ability to help these patients by speeding up lung development are hindered by a lack of understanding of human lung developmental biology. Here, we performed single cell RNA sequencing of the human fet...
ORGANISM(S): Homo sapiens 
2020-04-17 | E-MTAB-8221 | biostudies-arrayexpress
TranscriptomicsSingle-cell total RNA sequencing (VASA-seq) on index-sorted human colonic chip infected by wild-type S.Tm
We infected adult human intestine epithelium stem cell derived colonic chip with wild-type S.Tm 14028 with GFP label. To link intracellular replication levels to host response, we performed single-cell total RNA sequencing (VASA-seq) on index-sorted cells from GFPLow, GFPInt and GFPHigh levels.
ORGANISM(S): Homo sapiens 
2026-08-17 | E-MTAB-17486 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestinal ileum tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal ileum tissue samples from 3 individual biological specimens ages 11.4, 14.4, and 18.9 weeks post conception. The data set is composed of over 16,000 cells from diverse intestinal lineages.
ORGANISM(S): Homo sapiens 
2021-02-22 | E-MTAB-9906 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestine tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal duodenal tissue samples from 8 individual biological specimens across 7-21 weeks of gestation. The data set is composed of over 37,000 cells (between 3,000-9,000 cells per time point) from diverse intestinal lineages. Lineages ...
ORGANISM(S): Homo sapiens 
2020-12-12 | E-MTAB-9489 | biostudies-arrayexpress
TranscriptomicsMosaic perturbation of transcription factors in human retinal organoid development
To begin to understand how TFs regulate retinal cell type identity in human tissues, we established a pooled loss of function (LOF) experiment based on the CROP-seq protocol in developed retinal organoids. We targeted five TFs (OTX2, NRL, CRX, VSX2, and PAX6) that are important for retinal developme...
ORGANISM(S): Homo sapiens 
2023-02-24 | E-MTAB-12622 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of intestinal epithelial organoids derived from human fetal duodenum
Here, we used single-cell RNA-sequencing (scRNA-seq) to profile intestinal epithelial only organoids (also known as enteroids) from human fetal duodenum after one passage of in vitro growth. Organoids were grown in the standard 25% LWRN media with either 100 ng/ml of EGF or 1 ng/ml of EREG added.
ORGANISM(S): Homo sapiens 
2022-07-17 | E-MTAB-11912 | biostudies-arrayexpress
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