Flag-PFKFB3 was expressed in SW1990 cells. Immunoprecipitation analysis was performed using the anti-Flag antibody, and the extracts were analyzed by mass spectrometry.
Metabolomics of pancreatic cancer cell are modified by metabolic enzyme MTHFD2. SW1990 cells were transfected with MTHFD2 siRNA and control siRNA. Cells were collected for metabolomics analysis.
