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To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
ORGANISM(S): Mycolicibacterium Smegmatis Mc2 155 
2022-09-29 | PXD037081 |
MSCs were lysed in cell lysis buffer for Western blotting and IP (Beyotime, Cat. No. P0013). A Dynabeads™ protein G immunoprecipitation kit (Invitrogen, Cat. No. 10007D) was used for Co-IP according to the manufacturer’s instructions. Dynabeads were resuspended and placed on a magnet to remove the s...
ORGANISM(S): Homo Sapiens 
The IP-MS data includes SALL4 IP-MS data with or without BMP4 treatment at day 3 of JGES reprogramming, each group contain 3 repeats.
ORGANISM(S): Mus Musculus 
We enrolled PCa patients to analysis proteomics and build a survival prediction model
ORGANISM(S): Homo Sapiens 
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