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ProteomicsCHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is Q Exactive, part 2 of 3
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
ORGANISM(S): Homo sapiens (Human) 
2013-12-16 | PXD000533 | Pride
Chromesome 1 Q-exactive 8 Chpp Hcc cell lines 20 
ProteomicsCHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is AB5600, part 3 of 3
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-so...
ORGANISM(S): Homo sapiens (Human) 
2013-12-19 | PXD000535 | Pride
Chromesome 1 8 Chpp Hcc cell lines 20 Ab5600 
ProteomicsCHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, instrument is Q Exactive, part 1 of 3
The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-s...
ORGANISM(S): Homo sapiens (Human) 
2013-12-13 | PXD000529 | Pride
Chromesome 1 Q-exactive 8 Chpp Hcc cell lines 20 
ProteomicsDrosophila melanogaster proteome dataset with high coverage of the genome based on the LC-MS/MS platform
Here we describe a high coverage dataset of whole fly through a one-dimensional gel electrophoresis LC-MS/MS approach. By combining the datasets of two kinds of SDS-PAGE and two kinds of tagmata, the high coverage analysis resulted in the identification of 5,262 genes, corresponding to 38.23% of the...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2013-11-27 | PXD000455 | Pride
High coverage Proteome Lc-ms/ms 
ProteomicsDrosophila melanogaster iTRAQ HCD dataset
Quantitative comparation of the tagmata of body and head using iTRAQ based on HCD Fragmentation. The 8-plex iTRAQ Multiplex Buffer Kit (AB SCIEX, Foster City, CA) was used to label different peptide fractions, in which iTRAQ reagents dissolved in isopropyl alcohol. Another internal standard of D.mel...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2013-11-25 | PXD000454 | Pride
Hcd Itraq 
ProteomicsNear complete quantitative proteomics for Yeast
In this study, we attempted to achieve to date the most comprehensive coverage of the yeast proteome during log-phase growth and in the K11R mutant ubiquitin yeast strain with a combination of (1) extensive gel molecular weight fractionation and use of either of two digestion enzymes to improve cove...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-08-26 | PXD001928 | Pride
Yeast High coverage Silac Proteome Lc-ms/ms 
ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083957 | MassIVE
OtherThe Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins.
Data from ProteomeXchange, PXD ID: PXD002179. Experiment: CHPP_TESTIS_Tricine, file: CHPP_TESTIS_Tricine_2017.mgf. Published as part of J Proteome Res. 2015 . From the Abstract: {{i}} Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that prot...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310023526 | GPMDB
Resource Reanalysis 
Proteomics,MultiomicsHs_HCC_r1
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 1
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005189 | PeptideAtlas
Proteomics,MultiomicsHs_HCC_r2
CHPP chr 1,8,20 proteome dataset using the HCC cell lines of MHCC97H and HCCLM3, part 2
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005197 | PeptideAtlas
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