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The heated aldehyde-MPs solution was washed twice with NH₄HCO₃ (200 µL of 50 mM) buffer solution and then centrifuged (12,000 rpm, 5 min). Samples were alkylated with 5 μL of iodoacetamide for 1 h, subsequently undergoing tryptic digestion at 37 °C for 16 h (enzyme-to-protein ratio 1:50, w/w). The d...
ORGANISM(S): Bos Taurus 
2026-05-29 | PXD079095 |
Cell culture: H9 hESCs were grown using feeder-free conditions for 7 days after passage then either RNA was harvested or embryoid bodies were prepared, cultured in suspension for 4 days, plated on gelatin-coated plates, and cultured for an additional 9 days in embryoid body medium. Detailed protocol...
ORGANISM(S): Homo sapiens 
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