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GenomicsGenome-wide analysis of SPL7 regulated genes in Arabidopsis
To study the genes regulated by transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7) in Arabidopsis thaliana, we conducted chromatin immunoprecipitation-based sequencing (ChIP-seq) in 35S:FALG-SPL7 transgenic plant and spl7 mutant, and genome-wide sequencing-based transcript profiling...
ORGANISM(S): Arabidopsis thaliana 
2014-04-15 | E-GEOD-45213 | biostudies-arrayexpress
ProteomicsStress Granule-associated ZmCTU2 Confers Thermotolerance in Maize via Coordinated Regulation of Proteostasis and ROS Homeostasis
we performed comparative proteomic analysis of wild-type B104 and ZmCTU2-overexpressing lines. Seedlings at three-leaf-stage were grown under normal temperature condition (NC; 25°C for 12 days) and subsequently subjected to heat stress (HS; 43°C for 8 hours following NC growth). Using tandem mass ta...
ORGANISM(S): Zea Mays 
2025-12-17 | PXD072171 |
TranscriptomicsGenome-wide mapping of the HY5-mediated gene networks in Arabidopsis that involve both transcriptional and post-transcriptional regulations: RNA-Seq
LONG HYPOCOTYL 5 (HY5) is a basic leucine zipper transcription factor (TF) that functions downstream of multiple families of photoreceptors. Mutations in the HY5 gene cause a myriad of aberrant phenotypes in Arabidopsis, including elongated hypocotyl, reduced accumulation of pigments, halted chlorop...
ORGANISM(S): Arabidopsis thaliana 
2010-12-31 | E-GEOD-24970 | biostudies-arrayexpress
TranscriptomicsHuman acute myelogenous leukemia-initiating cells treated with fenretinide
Transcriptional profiling of human acute myelogenous leukemia (AML) CD34+ cells treated with 5 μM fenretinide. Two timepoints included are 6h, 12h, covering the apoptosis-induction time window of AML CD34+ cells responsing to the fenretinide treatment. We studied gene expression series in human AML ...
ORGANISM(S): Homo sapiens 
2011-11-30 | E-GEOD-33243 | biostudies-arrayexpress
TranscriptomicsDevelopment of a versatile, target-oriented tiling microarray assay for measuring allele-specific gene expression
In the study of gene expression regulation in plants, it is often desirable to distinguish transcript pools derived from different alleles present in the same organism. We report here an oligonucleotide tiling microarray designed to specifically target 518 single nucleotide polymorphisms (SNPs) betw...
ORGANISM(S): Oryza sativa 
2010-07-01 | E-GEOD-20678 | biostudies-arrayexpress
ProteomicsBRD4 regulates the osteogenic differentiation of MSCs
MSCs were lysed in cell lysis buffer for Western blotting and IP (Beyotime, Cat. No. P0013). A Dynabeads™ protein G immunoprecipitation kit (Invitrogen, Cat. No. 10007D) was used for Co-IP according to the manufacturer’s instructions. Dynabeads were resuspended and placed on a magnet to remove the s...
ORGANISM(S): Homo Sapiens 
2022-06-16 | PXD034615 |
ProteomicsMethylcobalamin protects against liver failure via engaging gasdermin E
To probe the binding sites of MeCbl on GSDME, 0.4 μM recombinant human GSDME (CSB-EP006766HU) purchased from CUSABIO (Wuhan, China) was incubated with 10 μM MeCbl in 100 μL buffer (20 mM HEPES, 150 mM NaCl and 50 μM DPA) at room temperature for 2 h and protected from light. 0.4 μM recombinant human ...
ORGANISM(S): Homo Sapiens 
2024-11-11 | PXD057768 |
ProteomicsChemoproteomic Mapping of the Glycolytic Targetome in Cancer Cells
we report a Target Responsive Accessibility Profiling (TRAP) approach for the global and quantitative analysis of glycolytic metabolites targetome in native cancer cell milieu.
ORGANISM(S): Homo Sapiens 
2020-11-17 | PXD022568 |
ProteomicsInvestigating the off-target effects of Pro-BA using label-free and TMTpro quantitative proteomics
Nevertheless, in-depth characterization of the proteome could reveal unknown off-targets of Pro-BA. To address this issue, we repeated the same experiment but extended MS analysis time to 2 hours in this revision, which will be elaborated in the next point. Nonetheless, the computed p-value of ALK p...
ORGANISM(S): Homo Sapiens 
2025-03-25 | PXD062245 |
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