This study was conducted by using Flag tag to IP proteins expressed with fusion small peptides encoding LncRNA MAGI2-AS3 and identified by high resolution mass spectrometry.
Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,00...
Aberrant sterol lipid metabolism is associated with physiological dysfunctions in the aging brain and aging-dependent disorders such as neurodegenerative diseases. There is an unmet demand to comprehensively profile sterol lipids spatially and temporally in different brain regions during aging. Here...