OBJECTIVE: Rheumatoid arthritis (RA) is a progressive disease including four stages, where gut microbiome is associated with pathogenesis. We aimed to investigate stage-specific roles of microbial dysbiosis and metabolic disorders in RA.
IP-MS assays of EDB(+)FN1 with a Flag epitope tag and EDB(-)FN1 with a His epitope tag. an equal amount of protein solution from each IP sample, the volume was adjusted to match the consistency of the original lysate.
