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ProteomicsKOPI - Kinase inhibitor proteome impact analysis
Kinase inhibitors often exhibit on/off-target effects, and efficient data analysis is essential for assessing these effects on the proteome. We developed a workflow for rapidly performing such a proteomic assessment, termed as kinase inhibitor proteome impact analysis (KOPI). We demonstrate KOPI’s ...
ORGANISM(S): Homo Sapiens (human) 
2022-06-16 | PXD029924 | JPOST Repository
MetabolomicsImaginal disc growth factor maintains cuticle structure and controls melanization in the spot pattern formation of Bombyx mori

The complex stripes and patterns of insects play key roles in behavior and ecology. However, the fine-scale regulation mechanisms underlying pigment formation and morphological divergence remain largely unelucidated. Here we demonstrated that imaginal disc growth factor (IDGF) maintains cuticle s...

2020-09-20 | MTBLS1825 | MetaboLights
ProteomicsPhosphosites of the yeast centrosome component Spc110 contribute to mitotic exit
Spc110 is an essential component of the spindle pole body (SPB), the yeast equivalent of the centrosome, that recruits the gamma-tubulin complex to the nuclear side of SPB to form the mitotic spindle. Here we identify two phosphosites (S11 and S36) in old (from maternal origin) Spc110. Yeast expres...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2023-04-03 | PXD033085 | JPOST Repository
ProteomicsK562 Chemical arrest cell cycle phase fractions 2D-MS-CETSA
K562 cells were synchronized by double thymidine block(DTB) based chemical arrest scheme into G1/S, S and Prometaphase(PM) fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC ...
ORGANISM(S): Homo Sapiens (human) 
2018-05-08 | PXD009146 | JPOST Repository
ProteomicsK562 Elutriation cell cycle phase fractions 2D-MS-CETSA
K562 cells were synchronized by cell size difference based Elutriation scheme into G1, S and G2 fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Meth...
ORGANISM(S): Homo Sapiens (human) 
2018-05-08 | PXD009171 | JPOST Repository
ProteomicsComplete sequences of the velvet worm slime proteins reveal that slime formation is enabled by disulfide bonds and intrinsically disordered regions
The slime of velvet worms (Onychophora) is a strong and fully biodegradable protein material, which upon ejection undergoes a fast liquid-to-solid transition to ensnare prey. However, the molecular mechanisms of slime self-assembly are still not well understood, notably because the primary structure...
ORGANISM(S): Cellular Organisms 
2022-09-20 | PXD031722 | JPOST Repository
ProteomicsCAMK2D recruits RNF8-MAD2 complex to RNA splicing proteins for mitotic checkpoint activation in GBM
Glioblastoma (GBM) is a lethal brain tumor with remarkable intra-tumoral heterogeneity and therapeutic resistance which is linked to glioma stem cell (GSC). GSC contains unstable genome and elevated genomic instability could impair GSC self-renewal and tumorigenicity. In addition, GSC displays great...
ORGANISM(S): Homo Sapiens (human) 
2023-07-21 | PXD038123 | JPOST Repository
ProteomicsDomain-specific p53 mutants activate EGFR by distinct mechanisms exposing tissue-independent therapeutic vulnerabilities
Mis-sense mutations affecting TP53 promote carcinogenesis both by inactivating its tumor suppressive functions, and by conferring aberrant pro-carcinogenic activities. We report here that mis-sense mutants in the p53 DNA-binding domain (DBD) and the transactivation domain (TAD) unexpectedly activate...
ORGANISM(S): Homo Sapiens (human) 
2023-02-17 | PXD031725 | JPOST Repository
ProteomicsPhosphoproteomic profiling of WNTinib
Phosphoproteome profiling small molecule on sensitive and insensitive murineHCC tumour organoids
ORGANISM(S): Mus Musculus (mouse) 
2023-03-16 | PXD024958 | JPOST Repository
UnknownGuidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Not available
S-EPMC4835977 | biostudies-literature
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