The rice genome encodes 18 HDACs from the three different families. The RPD3 family comprises 14 genes, the HDT and the Sir families contain each 2 genes. Previous studies showed that rice RPD3 members are involved in various developmental processes. In this work, we used quantitative proteomics to ...
Firstly, wild type seedlings that had germinated in root tubes for 14 days were taken to prepare protoplasts. The GCN5-FLAG transient expression vector was transformed into wild type protoplasts. The protoplasts were collected and purified by Flag beads. After quality testing of the samples, phospho...
Histone lysine acylations are regulated by primary metabolism in animal cells. However, histone non-acetyl acylation is not yet studied in plants that have distinct primary metabolic pathways. In this work, we detected rice histone lysine butyrylation (Kbu) and crotonylation (Kcr) sites by mass spec...
