Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MTAB-7276

ABSTRACT: Gene expression profiling of Fowlpox knock-out mutant viruses using Affymetrix GeneChip Chicken Genome Arrays

PROVIDER: E-MTAB-7276 | ExpressionAtlas | 2018-10-10

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MTAB-7276-analysis-methods.tsv	Tabular
	E-MTAB-7276-atlasExperimentSummary.Rdata	Rdata
	E-MTAB-7276-configuration.xml	Xml
	E-MTAB-7276-percentile-ranks.tsv	Tabular
	E-MTAB-7276.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 644

Similar Datasets

E-MTAB-3711

Project description:Identification of chicken Interferon Stimulated Genes (ISGs) using Affymetrix GeneChip Chicken Genome Arrays

2015-07-14 | E-MTAB-3711 | ExpressionAtlas

AIP inactivation leads to pituitary tumorigenesis through defective Gαi-cAMP signaling

Project description:Gene-level expression profiles of 7 Aip knock-out (triplicates from each) and 9 Aip wild-type mouse embryonic fibroblast cell lines were studied using Affymetrix GeneChip Mouse Exon 1.0 ST Arrays

2014-03-05 | GSE37927 | GEO

Mouse preimplantation development

Project description:We examined expression profiles of genes that could be regulated by the MAPK pathways during mouse preimplantation development. We used Affymetrix GeneChips for microarray analysis, and three independent experiments were carried out. 8-cell stage embryos were treated or untreated with MAPK inhibitors, and blastocyst stage embryos were recruited for the study. Hybridization was carried out with the GeneChip Mouse Expression Set 430 or GeneChip Mouse Genome 430 2.0 Array following Affymetrix instructions. The array was then washed and stained using the GeneChip fluidics station according to the manufacture's instructions. Hybridized arrays were scanned using an Affymetrix GeneChip Scanner. Expression analysis was performed using GeneChip Operating Software 1.0 (Affymetrix) and scaled to an average probe set intensity of 500. Keywords: other

2005-04-01 | GSE2229 | GEO

An increase in the abundance of Tim23 in Arabidopsis thaliana results in the disruption of complex I and activation of mitochondrial biogenesis.

Project description:Analysis of changes in global transcript abundance profiles of 2 week old tim23 OE (overexpressor) and tim23 KO (knock-out) mutant Arabidopsis plants complared to wild-type (Col-0) using Affymetrix GeneChipﾙ Arabidopsis ATH1 Genome Arrays.

2011-09-01 | E-MEXP-3309 | biostudies-arrayexpress

Gene expression in E10.5 Maxillary Arch Mesenchyme from control and Lhx6-/-;Lhx8-/- mutant embryos

Project description:We used laser capture microdissection to isolate maxillary arch mesenchyme from E10.5 embryos. This tissue was collected from both control (3x) and Lhx6-/-;Lhx8-/- mutant (3x) samples. Transcriptional profiling was performed using Affymetrix GeneChip Mouse Genome 430 2.0 arrays.

2015-07-23 | GSE71285 | GEO

Identification of putative targets of AP3/PI

Project description:To identify genes regulated by AP3/PI, we carried out microarray experiments using an Arabidopsis whole genome GeneChip array (ATH1 GeneChip, Affymetrix, Santa Clara, CA) in conjunction with an inducible AP3-GR system. For these experiments, we used 35S::AP3-GR transgenic plants in a 35S::PI, ap3-3 null mutant background for various dex or mock treatments. RNA was extracted from inflorescences at 0 and 4 hours after dex or a mock treatment and used as probes for our microarray experiments. Three biological replicates of each were hybridized to Affymetrix ATH1 arrays. We used the Affymetrix Microarray Suite software (MAS) to identify genes whose expression profiles changed only after dex-treatment and are likely targets of AP3/PI. Keywords: time course

2007-11-28 | GSE9702 | GEO

Comparison of microarray expression data from 22 day mouse germ cells from control and Mgat1 conditional knockout mice

Project description:Mechanistic insights into MGAT1 loss during spermatogenesis were investigated in germ cells from 22 day males. Gene expression changes induced by deletion of Mgat 1in spermatogonia were determined using the Affymetrix GeneChip Whole Transcript Plus Reagent Kit. We include the expression data obtained from control and conditional knock out Mgat1 mutant mouse testis germ cells . We identified the genes that were differentially expressed (DEGs) in 22 day Mgat1 mutant versus control germ cells, with a significance level of ANOVA p < 0.05 and an absolute fold-change (linear) less than -2 and greater than 2.0.

2018-02-13 | GSE99033 | GEO

Gene expression profiling of Fowlpox knock-out mutant viruses using Affymetrix GeneChip Chicken Genome Arrays

Project description:Fowlpox virus (FWPV) is a double-stranded DNA virus, used as a live vaccine against poultry diseases, and considered a promising potential mammalian vaccine vector. Similar to mammalian poxviruses, FWPV has evolved mechanisms to evade host immune responses at different levels. We infected chicken embryo fibroblasts (CEF) with individual FWPV mutants (n=59), each deficient in one non-essential gene, from a previously described knock-out virus library [Laidlaw et al, 2013 J Virology 87(9); Laidlaw and Skinner, 2014 Bio-protocol 4(10); e1126]. Host responses to the mutant viruses were screened at 16h post infection for each virus using Affymetrix GeneChip Genome arrays which includes probes for most FWPV genes. Controls included the wild type virus and uninfected samples. To avoid systematic errors due to different batches of CEF and hybridisation/scanning on different dates, samples were processed in groups of randomised samples.

2019-01-01 | E-MTAB-7276 | biostudies-arrayexpress

Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

Project description:Relative expression levels of mRNAs in chicken cecal epithelia experimentally infected with Eimeria tenella were measured at 4.5 days post-infection. Two weeks old chickens were uninfected (negative control) or were orally inoculated with sporulated oocysts of Eimeria tenella. Cecal epithelia samples were collected from >12 birds in infected or uninfected group at 4.5 d following infections, in which samples from 4 birds were pooled together to form a total 3 biological replicates in each group. Parasite merozoites were also collected from four infected chickens at 5 d after infections. Uninfected control samples, merozoites and infection group samples were selected for RNA extraction and hybridization on Affymetrix microarrays. We used Affymetrix GeneChip chicken genome arrays to detail the chicken cecal epithelia gene expression in the control and E. tenella-infected birds.

2012-12-10 | GSE39602 | GEO

Drosophila melanogaster

Project description:Explaining Differences in Saturation Levels for Affymetrix GeneChip Arrays

| PRJNA98415 | ENA