Transcription profiling of community-acquired invasive and nasal carriage S.aureus isolates to identify genes associated with invasion, genomic variability and population structure

ABSTRACT: Staphylococcus aureus is the most common cause of hospital-acquired infection. In healthy hosts outside of the health care setting, S.aureus is a frequent colonizer of the human nose but rarely causes severe invasive infection such as bacteremia, endocarditis, or osteomyelitis. To identify genes associated with community-acquired invasive isolates, regions of genomic variability, and the S.aureus population structure, we compared 61 community-acquired invasive isolates of S.aureus and 100 nasal carriage isolates from healthy donors using a microarray spotted with PCR products representing every gene from the seven S.aureus sequencing projects. The core genes common to all strains were identified, and 10 dominant lineages of S.aureus were clearly discriminated. Each lineage carried a unique

INSTRUMENT(S): Ultracentrifuge, Spectronic Helios Alpha UV-Vis spectrophotometer

ORGANISM(S): Staphylococcus aureus

SUBMITTER:  

PROVIDER: E-BUGS-33 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress