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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human monocytic cell line, THP-1 cells were stimulated 4 hours with chemokine MIG or CCL2/MCP-1

ABSTRACT: The objective of the study was to discover new functions of chemokine CXCL9/MIG, a cationic chemokine that has antimicrobial properties. Human monocytic cell line, THP-1 cells were stimulated 4 hours with chemokine MIG or CCL2/MCP-1 (a chemokine that is not positively charged and has no antimicrobial activity). By using a 21,000 oligo-based DNA human microarray we analyzed gene expression profiles induced by MIG and by MCP-1. The gene expression profile induced by MIG was >85% different from that induced by MCP-1. MIG increased transcription of genes encoding various chemokines (including CCL3/MIP-1alpha, CCL4/MIP-1beta, CCL2/MCP-1 and CXCL8/IL-8), cytokines (TNF-alpha), indicating that MIG has an immunomodulatory effect on THP-1 cells. Additionally, MIG strongly up-regulated a set of genes identified as having tumor suppressor functions, including BTG2, BTG1, P21, IGFBP3, DSCR1 and genes encoding markers for mature leukocyte differentiation (PLAU, LPL, PLAUR etc). In parallel, MIG down-regulated the expression of oncogenes (MYC), and genes related to cell proliferation and cell cycle progression. This gene profile strongly suggested that the chemokine MIG had anti-proliferative activity and might induce THP-1 cells to undergo terminal differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Jiang-Hong Gong

PROVIDER: E-FPMI-5 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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