Genome-wide profiling of salt fractions maps physical properties of chromatin

ABSTRACT: We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80mM or 150mM NaCl after digestion contain predominantly mononucleosomes and represent calssical 'active' chromatin. Profiles of these low-salt-soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of RNA polymerase II. Nearly quantitative recovery of chromatin is obtained with 600mM NaCl, however, the remaining insoluble chromatin is enriched in actively transcribed regions. Salt-insoluble chromatin likely represents oligonucleosomes that are attached to large protein complexes. Both low-salt extracted and insoluble chromatin ar

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jorja Henikoff 

PROVIDER: E-GEOD-13217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress