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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Whole genome transcription profile of antigen receptor activated B cells

ABSTRACT: To search for rapid changes in gene expression following BCR activation, we performed DNA microarray analysis of activated splenic B cells with and without anti-IgM treatment for 3 hour. The expression of a remarkably large set of genes differed significantly. Primary B-lymphocytes were purified from mouse spleens and stimulated with IL-4 (Pepro-Tech) and CD40L (R&D Systems) as indicated at 5 ng/ml and 200 ng/ml, respectively. The BCR of stimulated cells was activated by incubation with goat F(abâ)2 anti-mouse IgM (Southern Biotechnologies) at 2.5 Î¼g/ml for 3 hour.

ORGANISM(S): Mus musculus

SUBMITTER: Thomas GrundstrÃ¶m

PROVIDER: E-GEOD-15606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Initiation of antigen receptor-dependent differentiation into plasma cells by calmodulin inhibition of E2A.

Hauser Jannek J Verma-Gaur Jiyoti J Wallenius Anders A Grundström Thomas T

Journal of immunology (Baltimore, Md. : 1950) 20090624 2

Differentiation of B lymphocytes into Ab-secreting plasmablasts and plasma cells is Ag driven. The interaction of Ag with the membrane-bound Ab of the BCR is critical in determining which clones enter the plasma cell response. However, not much is known about the coupling between BCR activation and the shift in transcription factor network from that of a B cell to that of ASC differentiation. Our genome-wide analysis shows that Ab-secreting cell differentiation of mouse B cells is induced by BCR ...[more]

PMID: 19553523

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