Transcription orofiling of Saccharomyces cerevisiae upon exposure to aaxitoxin

ABSTRACT: Saxitoxin is a potent neurotoxin produced by several species of dinoflagellates and cyanobacteria. The molecular target of saxitoxin in higher eukaryotes is the voltage-gated sodium channel; however, its target in lower eukaryotic organisms remains unknown. The goal of this study was to obtain the transcriptional fingerprint of the model lower eukaryote Saccharomyces cerevisiae upon exposure to saxitoxin to identify potential genes suitable for biomarker development. Microarray analyses identified multiple genes associated with copper and iron homeostasis and sulfur metabolism as significantly differentially expressed upon exposure to saxitoxin; these results were verified with quantitative reverse-transcriptase PCR (qRT-PCR). Additionally, the qRT-PCR assays were used to generate expressi

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Kathleen Cusick 

PROVIDER: E-GEOD-16652 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress