GSK-3β inhibition promotes engraftment of ex vivo expanded hematopoietic stem cells

ABSTRACT: Glycogen synthase kinase-3β (GSK-3β) has been recently identified as an important regulator of stem cell function. In vitro studies show that GSK-3β inhibition delays proliferation of human haematopoietic progenitor cells while increasing numbers of late dividing multipotent progenitors. Gene expression analysis revealed that GSK-3β inhibition modulates the expression of a subset of genes that are transcriptional targets for cytokines. GSK-3β inhibition antagonised down-regulation of genes encoding cyclin dependent kinase inhibitor p57 and a member of the growth arrest and DNA damage 45 family, GADD45B as well as up-regulation of cyclin D1 by cytokines, providing a possible mechanism for the BIO-induced delay in cell cycle progression. Surprisingly, inhibition of GSK-3β earlier show

ORGANISM(S): Homo sapiens

SUBMITTER: Kap-hyoun Ko 

PROVIDER: E-GEOD-21073 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress