Time Course Gene Expression Microarray Analysis of a Mouse B-cell Line (CH12.LX) Activated with Lipopolysaccharide and Treated with 2,3,7,8-Tetrachlorodibenzo-p-dioxin

ABSTRACT: The objective of the study was to characterize gene expression cascade involved in the suppression of B-cell activation and differentiation by 2,3,7,8-tetrachlorodibenzo-p-dixoxin (TCDD). The underlying hypothesis was that multiple nodes in the B-cell differentiation network are directly or indirectly regulated by TCDD through its receptor, the AHR. The mouse B-cell line (CH12.LX) was plated at 1X10^5 cells/ml at time 0 and activated with lipopolysaccharide (LPS, Salmonella typhosa). The cells were then treated with either dimethyl sulfoxide (DMSO, 0.01%) or 10 nM 2,3,7,8-tetrachlorodibenzo-p-dixoxin (TCDD). The cells were harvested at 0, 8, 12, 24, 36, and 48 hrs post-treatment. At the 0 hr time point, cells were untreated. A total of 4 experimental replicates per time point per treatme

ORGANISM(S): Mus musculus

SUBMITTER: Russell Scott Thomas 

PROVIDER: E-GEOD-21272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress