Gene expression analysis in non-failing and failing myocardium pre and post pulsatile and non-pulsatile VAD support

ABSTRACT: Mechanical unloading by ventricular assist devices (VAD) leads to significant gene-expression changes often summarized as reverse remodeling. However, little is known on individual transcriptome changes during VAD-support and its relationship to non-failing hearts (NF). In addition no data are available for the transcriptome regulation during non-pulsatile VAD-support. Therefore we analysed the gene-expression patterns of 30 paired samples from VAD-supported (including 8 non-pulsatile VADs) and 8 non-failing control hearts (NF) using the first total human genome-array available. Transmural myocardial samples were collected for RNA-isolation. RNA was isolated by commercial methods and processed according to chip-manufacturer recommendations. cRNA were hybridized on Affymetrix HG-U133 Plus 2

ORGANISM(S): Homo sapiens

SUBMITTER: Sonja Steppan 

PROVIDER: E-GEOD-21610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress