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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from mesothelial (LP9) and mesothelioma cells (HMESO) inhibited for extracellular-regulated kinase (ERK) 1, 2, or 5

ABSTRACT: Extracellular-regulated kinases (ERK1/2 and 5) are known to play important roles in growth and drug resistance of various cancers. Here we show roles of inhibition of ERK1, ERK2, or ERK5 on gene expression profiles of epithelioid malignant mesothelioma (MM) cells (HMESO). Untransfected human mesothelial cells (LP9), MM cells (HMESO) or HMESO cells stably transfected with shControl, shERK1, shERK2 , or shERK5 plasmids were used. Microarrays were performed on samples from 3 independent experiments. Each sample was analyzed on a separate array (3 independent biological replicates).

ORGANISM(S): Homo sapiens

SUBMITTER: Arti Shukla

PROVIDER: E-GEOD-21750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Blocking of ERK1 and ERK2 sensitizes human mesothelioma cells to doxorubicin.

Shukla Arti A Hillegass Jedd M JM MacPherson Maximilian B MB Beuschel Stacie L SL Vacek Pamela M PM Pass Harvey I HI Carbone Michele M Testa Joseph R JR Mossman Brooke T BT

Molecular cancer 20101215

<h4>Background</h4>Malignant mesotheliomas (MM) have a poor prognosis, largely because of their chemoresistance to anti-cancer drugs such as doxorubicin (Dox). Here we show using human MM lines that Dox activates extracellular signal-regulated kinases (ERK1 and 2), causally linked to increased expression of ABC transporter genes, decreased accumulation of Dox, and enhanced MM growth. Using the MEK1/2 inhibitor, U0126 and stably transfected shERK1 and shERK2 MM cell lines, we show that inhibition ...[more]

PMID: 21159167

Publication: 1/2

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