In vitro carcinogenicity testing with Balb/c 3T3 Cells treated with various chemical carcinogens

ABSTRACT: Background: Information on the carcinogenic potential of chemicals is only availably for High Production Volume products. There is however, a pressing need for alternative methods allowing for the chronic toxicity of substances, including carcinogenicity, to be detected earlier and more reliably. Here we applied advanced genomics to a cellular transformation assay to identify gene signatures useful for the prediction of risk for carcinogenicity. Methods: Genome wide gene expression analysis and qRT-PCR were applied to untransformed and transformed Balb/c 3T3 cells that exposed to 2, 4-diaminotoluene (DAT), benzo(a)pyrene (BaP), 2-Acetylaminoflourene (AAF) and 3-methycholanthrene (MCA) for 24h and 120h, at different concentrations, respectively. Furthermore, various bioinformatics tools we

ORGANISM(S): Mus musculus

SUBMITTER: Jens Linge 

PROVIDER: E-GEOD-22180 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress