Phyloepigenetic comparison of apes

ABSTRACT: We have determined methylation state differences in the epigenomes of neutrophils purified from human and chimpanzee. We used deep sequencing of ends generated by digestion with a methylation-sensitive restriction enzyme, followed by analysis with the MetMap computational pipeline to infer methylation states from the sequencing data. Using the orangutan as an outgroup, analysis of DNA sequence substitutions in CG-dense regions that are either methylated or unmethylated in all three species indicates that methylation states in the neutrophil reflect methylation states in the germline. Differences in methylation states were not correlated with differences in the local genomic sequences, indicating that they can be determined independently of local DNA sequence. Methylation differences were n

ORGANISM(S): Pongo pygmaeus

SUBMITTER: Dario Boffelli 

PROVIDER: E-GEOD-22376 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress