Representative Illumina sequencing libraries upon T7-polymerase based amplification

ABSTRACT: This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing Adaptation of standard Illumina sequencing protocol to obtain representative sequencing libraries after sample amplification. Comparing different amplification methods with amplification free sequencing.

ORGANISM(S): Plasmodium falciparum 3D7

SUBMITTER: H. Stunnenberg 

PROVIDER: E-GEOD-23868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress