Comparative Genome Hybridization between Candida albicans and Candida dubliniensis and their derivatives

ABSTRACT: Total genomic DNAs of CAKS20, CdUM4b, the pseudotetraploid hybrid strain CACD1 and the progeny hybrid strain Pre3 were isolated. In an attempt to determine the number of copies of individual chromosomes, we used comparative genome hybridization (CGH) analysis using specially designed whole genome microarrays representing C. albicans and C. dubliniensis genomes. The arrays contained 60 mer probes that fell in three categories either C. albicans specific or C. dubliniensis specific or common to both. The specific probes were expected to work as internal controls when hybridized to parental genomic DNA alone. The arrays were individually hybridized to Cy3-labeled genomic DNA from either of C. albicans (CAKS20), C. dubliniensis (CdUM4b) parents or the somatic psudotetraploid hybrid strain, CA

ORGANISM(S): Candida albicans

SUBMITTER: Kaustuv Sanyal 

PROVIDER: E-GEOD-24269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress